BD Accuri™ C6 Cytometer Flow cytometry within reach.™

Flow Cytometry within Reach The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. As a result, flow cytometry has become a staple of modern laboratories around the world. Innovations in ease of use reflected in the BD Accuri C6 cytometer make these powerful capabilities more accessible to a new generation of flow cytometry users. The...

Pre-Optimized Detectors, Minimized Setup Time The system is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. Optional filters and the Selectable Laser Module expand the available fluorochrome combinations. During manufacture, laser and optical alignments are set and locked down. The result is that each BD Accuri C6 cytometer is manufactured with standardized fluorescence...

Sample Flexibility with Optional Walkaway Sample Loading A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter. The non-pressurized system supports any brand of 12 x 75-mm (or smaller) sample tubes, including microcentrifuge tubes and tubes made of polypropylene or polystyrene. The BD Accuri C6 cytometer simplifies system maintenance with automatic...

Intuitive Software—Master in Minutes Sample data can be customized in the Statistics tab. Data is displayed in a master table, and statistics can be easily copied and pasted into spreadsheets to facilitate reporting. To simplify creating presentations, plots can be imported into Microsoft® Office applications using drag and drop. BD Accuri C6 software supports live gating, event coloring, export of publication-quality, vector-scalable graphics, and batch analysis, for review or modification of multiple samples for the automatic creation of Microsoft PowerPoint® and Excel files. BD Accuri C6 software...

SOFTWARE Analyze tab A 800 800 800 600 600 600 Neg Neg Neg control control control Count 500 Count Count 500 Count 500 Partial data displayed Partial data displayed Partial data displayed TRA-1-60 TRA-1-60 TRA-1-60 Partial data displayed Partial data displayed Partial data displayed Neg Neg Neg SSEA-4 SSEA-4 control SSEA-4 control control CD147 CD147 CD147 500 Neg Neg Neg control control control Count Count 500 Count 500 Oct 3/4 3/4 Oct 3/4 Oct 0 0 0 0 0 0 SSEA-1 SSEA-1 SSEA-1 0 0 0 The human embryonal carcinoma (EC) cell line 2102Ep can serve as a reference standard for human embryonic and induced...

APPLICATIONS Easy to Learn and Use— Fits a Wide Range of Applications Multiplexed soluble protein quantitation With BD™ Cytometric Bead Array (CBA) reagents, the BD Accuri C6 flow cytometer, and FCAP Array™ analysis software, users can quantify multiple proteins simultaneously, using the broad dynamic range of fluorescence detection offered by flow cytometry and antibody-coated beads. This method significantly reduces sample volume requirements and time to results in comparison with traditional ELISA and Western blot techniques. BD CBA Kits take advantage of the standard BD Accuri C6 configuration....

Fluorescent proteins have come a long way since the original application of Green Fluorescent Protein (GFP) for the detection of gene expression. Fluorescent proteins now span the entire spectrum from short violet to long red, and can be used to study a wide variety of cellular phenomena. 10 4.1 0 3 3 10 10 4 4 10 10 5 5 10 10 6 6 10 10 3 103.4 3 103.4 10 10 2 10 1 10 2 10 SSC-H SSC-H 1 10 10 2 10 2 3 10 10 FSC-H 0 10 10 1 10 10 2 10 10 3 10 10 4 4 10 10 FL1: 530 BP 5 5 10 10 6 6 10 10 10 10 2 P2 P2 54.9% 54.9% 10 10 1 SSC-H 2,000 Count 1,000 41956 / 209780 (20%) 41956 events displayed events...

Special Order Products for Unique Needs The BD special order program allows customers to purchase instruments configured to fit precise research and assay needs. This innovative program is tailored to the special needs of research at the leading edge of biomedical discovery, and offers a wide range of choices to help researchers create the ultimate customized instrument for their requirements. Analysis of DNA content and ploidy in plants Data courtesy of David W. Galbraith, School of Plant Sciences and Bio5 Institute for Collaborative Bioresearch, University of Arizona, Tucson, AZ, USA. 12 Your...

CUSTOM CONFIGURATION 101 102 103 104 105 106 107.2 101 102 103 104 105 106 107.2 Chlorophyll a,b fluorescence Chlorophyll a,b fluorescence FL3 ex: 488; em: 670 LP FL3 ex: 488; em: 670 LP Site 1 Site 1 P7 P7 50.0% 50.0% P6 P6 42.8% 42.8% 101 102 103 104 105 106 107.2 101 102 103 104 105 106 107.2 101 102 103 104 105 106 107.2 101 102 103 104 105 106 107.2 The BD Accuri C6 can help speed sample analysis for biologists studying marine and freshwater ecosystems. The system is portable enough to travel aboard ship and can handle particles as small as 0.5 μm, quickly delivering multiparametric measurements...

SERVICES Services and Support BD Biosciences is fully committed to the success and satisfaction of its customers and offers a range of options for BD Accuri support. Fast, easy installation The BD Accuri C6 cytometer can be customer installed within just an hour of taking it out of the box. A step-by-step quick start guide and online video simplify installation. Preventative maintenance Preventative maintenance procedures should be performed every two months to change the sheath filter, pump tubing, and fluidic filters, and to clean the SIP. Technical application support Our technical application...