BD Cytometric Bead Array
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BD Cytometric Bead Array Multiplexed Bead-Based Immunoassays

Cover Illustration: Fairman Studios, LLC.

BD Cytometric Bead Array: Multiplexed Bead-Based Immunoassays BD™ Cytometric Bead Array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. The BD CBA system uses the broad dynamic range of fluorescence detection offered by flow cytometry and antibody-coated beads to efficiently capture analytes. Each bead in the array has a unique fluorescence intensity so that beads can be mixed and run simultaneously in a single tube. This method significantly reduces sample requirements and time to results in comparison with traditional ELISA and Western...

BD CBA Flex Sets— Open and Configurable Soluble protein assays BD CBA Soluble Protein Flex Set assays are available for the detection of cytokines, chemokines, and growth factors from serum, plasma, or tissue culture supernatant samples. These include T-cell differentiation factors, modulators of inflammation, and other key markers for monitoring the immune response. The BD CBA Flex Set system provides an open and configurable menu of bead-based reagents designed to make it easy to create multiplex assays. Available specificities include soluble protein assays for detection of human, mouse,...

OPEN AND CONFIGURABLE BD CBA 30-plex assay 5 Enhanced sensitivity assays The BD CBA Enhanced Sensitivity Flex Set assays provide a new level of detection compared to the traditional BD CBA Flex Set assays. The Enhanced Sensitivity Flex Sets can detect as low as 0.274 pg/mL in a multiplexed assay. This breakthrough allows researchers to get quantitative results in samples that were previously below the level of detection. The system includes assays for cytokines involved in inflammation and leucocyte differentiation, which can be combined to create a multiplexed panel. The system provides a...

Build a Multiplex Step-by-Step BD CBA Flex Sets make it easy to build a multiplex by following five simple steps. The finished assay can be acquired on a variety of dual-laser flow cytometers and analyzed using FCAP Array™ software. Instrument Reporter Parameter Clustering Parameters BD FACSVerse™ flow cytometer BD Accuri™ C6 flow cytometer* BD FACSCanto™ II flow cytometer BD FACSAria™ III cell sorter BD FACSCalibur™ flow cytometer FCAP Array Parameters for BD CBA Flex Sets *Requires the BD Accuri Selectable Laser Module (Cat. No. 653126) STEP 1: Choose from our menu of Human, Mouse, Rat,...

MAXIMUM FLEXIBILITY STEP 3: Perform the assay following the instructions in the (A) Master Buffer Kit manual. Final Concentration Final Concentration (units/mL) (units/mL) Con EGF STEP 4: Acquire samples on a dual-laser flow cytometer. p-ERK BD CBA Flex Set reagents have been verified for performance 1400.00 on a number of BD dual-laser flow cytometry platforms. (B) STEP 5: Analyze data files using FCAP Array multiplex analysis software. Use the intuitive FCAP Array software to plot standard curves and calculate sample concentrations. Analysis of phospho-ERK1/2 protein levels in HEK 293 and...

BD CBA Kits: Consistent Results with Routine Panels BD CBA Kits provide preconfigured panels for ultimate ease of use. These kits enable multiplex analysis of complex biological samples on a flow cytometer. In contrast to BD CBA Flex Sets, the kits are preconfigured by functional areas of biology (eg, Th1/Th2 or inflammatory cytokines) to measure up to seven analytes simultaneously using capture beads that contain unique amounts of a single red dye. The unique spectral properties of this dye enable analysis of samples on flow cytometers that have a single 488-nm laser or on dual-laser...

Comparison of BD CBA Standards to the NIBSC/WHO International Standards The NIBSC protein standards are recognized by the World Health Organization (WHO) as international biological standards. They meet established requirements for accuracy, consistency, and stability. The NIBSC/WHO standards are assigned potency values in International Units (IU) of biological activity and nominal mass (ie, not absolute mass values). Therefore they cannot be used to establish absolute concentrations for a cytokine preparation. However, these standards do provide a means to facilitate comparisons of...

Simplify Analysis with FCAP Array v3.0 Software With FCAP Array software (v3.0), sample results are obtained just minutes after performing a CBA experiment. After following the assay protocol, simply collect your data on a flow cytometer, export FCS data files, and then analyze the data using FCAP Array software. Acquire data on a flow cytometer Analyze in FCAP Array software FCAP Array v3.0 software • Intuitive layout of samples and assignment to data files • Standard dilution calculator for fast data entry • Debris filtering and manual clustering options • Results viewing by sample or...

Users of the BD FACSVerse flow cytometer equipped with BD FACSuite™ software will enjoy many automated features for even faster results. Based on keywords that are entered prior to acquisition and read by FCAP Array software, analysis begins as soon as the FCS data files are identified. These keywords include identification of sample type (eg, standard, test sample, or control), identification of a plex name that automatically assigns a plex if a match is found in the FCAP Array plex library, and the sample dilution factor. This automated workflow also reads the Sample ID and Experiment...

Additional Options to Enhance Flexibility BD CBA Functional Beads to conjugate for unique requirements BD CBA Flex Set Functional Beads are unconjugated beads that allow researchers to conjugate their own antibody or protein of interest using sulfo-SMCC chemistry. The conjugation procedure takes less than four hours using common laboratory supplies along with the buffers in the BD CBA Functional Bead Conjugation Buffer Kit. This is an ideal platform for converting existing ELISAs into bead-based immunoassays that can be mixed with our portfolio of BD CBA Flex Set assays. The availability of...