BD FACSAria III Cell Sorter ™

Innovation is built into the BD FACSAria III The BD FACSAria™ III cell sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary ease-of-use that has led to the unparalleled success of the BD FACSAria™ cell sorter. Since the introduction of the first BD FACSAria in 2003, each successive generation has opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. Now, the BD FACSAria III system is even more powerful, dependable, and easy to use. Here are a few of the new innovations that the...

Proven dependability and ease-of-use put the system in a class of its own Sensitivity for multicolor and sorting applications The fluidics system in the BD FACSAria III cell sorter is pressure driven. Positive air pressure forces sample cells through an optically gel-coupled cuvette flow cell. Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point. Gel-coupled cuvette flow cell At the heart of the BD FACSAria III is a quartz cuvette flow cell in true fixed alignment with the laser and gel coupled...

Nozzles for a range of particles A choice of nozzles lets users sort a wide range of particle sizes. Nozzles are available in four sizes: 70, 85, 100, and 130 microns. Nozzles are readily accessible and easy to change, with a design offering tight registration for a secure fit. This means a reproducible drop profile after every nozzle exchange, resulting in reproducible instrument setup and alignment. The software sort setup matches pressure and sort settings to the nozzle being used. Easy aseptic setup and cleaning Innovations in the fluidics system such as easy-to-insert nozzles, automated...

Fluidic system improvements are built in to make the system easier and safer to operate Engineered into the system Fluidics cart A self-contained fluidics cart supplies sheath and cleaning fluids and collects waste from the cytometer. The cart also provides the air pressure and vacuum needed to achieve pressure from 5 to 75 psi, to accommodate a variety of cell sorting applications. BD FACSDiva™ software adjusts the air pressure. The fluidics cart is typically positioned directly under or to the left of the cytometer. The fluidics cart holds a 10-L stainless steel sheath tank, a 5-L stainless...

Sample injection chamber During acquisition, the sample injection chamber is pressurized, forcing the sample to the cuvette flow cell. To simplify acquisition, the chamber temperature and agitation settings are controlled using BD FACSDiva software. A variety of tube holders are provided, from 15-mL centrifuge tube to 1.0-mL microtube size. To minimize clogging, 35- and 50-micron sample line filters are available. From the sort block to the collection chamber After leaving the nozzle, particles pass through the sort block that houses the deflection plates. The novel design fixes the plates in...

Sensitivity and resolution beyond compare Increased efficiency for multicolor detection Innovations in the optical system, pioneered by BD, efficiently maximize signal detection and greatly increase sensitivity and resolution for each color in a multicolor assay. Enhanced sensitivity and resolution mean that even dim populations can be readily identified and sorted. The optics system allows optimizing multicolor assays and panel design for superior results. The design allows choice of laser excitation wavelength(s) that illuminate cells in the sample, and collection optics that direct light scatter...

Trigon and octagon detector arrays Precision optical design The many innovations in the BD FACSAria III’s optical system, such as the patented gel-coupled cuvette and octagon detection system, and the 9 μm x 65-μm beam spot, are designed to work together to maximize sensitivity and resolution. This precision design delivers a more efficient optical system enabling the use of lower powered lasers, which in turn reduces the total cost of instrument operation. Relative Cell Number 95.8 Relative Cell Number Representative FoxP3 staining of sorted Tregs To determine purity, as defined by FoxP3+ status,...

The latest state-of-the-art is available with a simple upgrade Multicolor flexibility Lower cost of ownership The unique, efficient design of the optical system delivers a lower cost of operation than stream-in-air sorters. The BD FACSAria III delivers maximum sensitivity and resolution using fiber launched solid state lasers. No special power or cooling is needed for these lower powered air cooled lasers. Tube: HeLa Population All Events Scatter SSC gate FSC gate P1 P2 CFP 405 Transfected-HeLa Tube: HeLa Population All Events Scatter SSC gate FSC gate P1 P2 CFP-transfected HeLa cells The 445-nm...

New X-mount Optical Plate Side population As a control, side population expression was blocked (right dot plot). Cancer cell line side population Human HT-29 colon cancer cells were stained with Hoechst 33342 and acquired on the BD FACSAria III equipped with a 375-nm laser (left dot plot). Doublet Doublet-CD45+ Lineagec-Kit+ Sca-1 + LinSide population c-Kit+ Sca-1 + Lin- AND Side population Tube: mouse BM CD34KSL Hoechst 5 *0ug/ml Antibodies used were c-Kit PE, Sca-1 PE-Cy™7, Lineage FITC, CD34 APC, CD45 APC-Cy7, PI, Hoechst Blue, and Hoechst Red. The SP fraction (red) was limited to those cells...

BD FACSDiva software helps you move from analysis to sorting Compatible with other BD analyzers and sorters BD FACSDiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the BD FACSAria III workstation. BD FACSDiva software is common across many BD cell analyzers and cell sorters, including BD FACSCanto and BD™ LSR systems. Researchers gain application flexibility because it is easier to move the assay design and optimization to another platform, for example, from analysis to sorting. The Cytometer Setup and Tracking (CS&T) feature of BD FACSDiva software...