BD FACSCanto II
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A proven platform for maximum reliability and the highest quality results

A proven platform for maximum reliability and the highest quality results Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high quality results. The BD FACSCanto II provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors. The BD FACSCanto II features many innovations. At the heart of the cytometer, for example, the fluidics system features a fixed alignment flow cell to...

Saves time, improves reproducibility High Performance, Innovative System The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate. A fluidics...

Flow cell LED alerts LEDs are located in the front door to monitor each acquisition parameter. Each LED blinks when the signal level reaches a prescribed threshold.

Maximize multicolor performance Innovative Optical Design Delivers Highest Sensitivity The innovative designs for both the excitation and collection optics reduce excitation losses, yielding more information from each sample. The optics of the BD FACSCanto II system consist of an excitation source with up to three lasers, a blue (488-nm, air-cooled, 20-mW solid state), a red (633-nm, 17-mW HeNe) and a violet (405-nm, 30-mW solid state). Laser excitation optics illuminate cells in the sample and collection optics direct light scatter and fluorescence signals through spectral filters to the...

Excitation optics focus the laser beams Emission spectra of commonly used fluorochromes Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences The BD FACSCanto II system is designed for these fluorophore combinations. BD FACSCanto II flow cytometry system Green Yellow Orange Red Infrared Red Far Red Infrared Green Blue FITC PE PE-Texas Red®† PerCP PE-Cy7 APC Alexa Fluor® 700† APC-Cy7 AmCyan† BD Horizon™ V450 PerCP-Cy5.5 Alexa Fluor® 647 BD APC-H7 Pacific

Productivity support Options and Related Tools to Support Lab Requirements Sample introduction productivity can be gained with the optional BD FACS Loader or the BD High Throughput Sampler (HTS). BD High Throughput Sampler (HTS)* Designed for research use, the optional BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition that can speed through a microtiter plate in less than 15 minutes, with less than 1% sample carryover. In high-throughput mode, fast acquisition speed is achieved by synchronizing two high-precision pumps for sample mixing, sample...

BD FACS Loader The BD FACS Loader is an instrument option that allows walkaway sample introduction to further improve productivity. The BD FACS Loader carousel accommodates up to 40 12x75-mm tubes and automatically loads them on the BD FACSCanto II system without operator intervention. Mounted directly on the cytometer, the device includes a drive system, a tube lifter mechanism, and sensors on the sliding drawer. Two sliding doors safely enclose the drawer to protect technicians from moving parts during operation. The BD FACS Loader utilizes compressed air to allow a more reliable tube...

Sample prep, setup, and analysis A Turnkey System for Clinical Use Designed to address the needs of today’s busy clinical lab, the BD FACSCanto II has a proven track record of reducing hands-on time and improving reliability of results. A high degree of automation and quality control helps save time, reduce cost, and improve reproducibility of results. Cytometer settings are tracked over time by Levey-Jennings graphs to monitor cytometer performance. Adjustments are made automatically to ensure consistent data integrity and reproducible results from day to day and experiment to experiment....

The BD HLA-B27 module for the BD FACSCanto II system provides a complete system for rapid detection of HLA-B27 antigen, which is clinically relevant to the evaluation of seronegative spondyloarthropathies. Gated Events Preset HLA-B27 Marker Sample HLA-B27 Median Conclusion

Versatile, easy-to-use A Powerful Platform for Research Use The BD FACSCanto II system brings BD flow cytometry and multicolor analysis to a new level to help researchers meet complex challenges and advance their research. BD FACSDiva Software for Setup, Acquisition, and Analysis BD FACSDiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the BD FACSCanto II workstation. BD FACSDiva operating software is common across many BD cell analyzers and cell sorters, including BD™ LSR and BD FACSAria™ systems, giving researchers application flexibility....

Lysed whole human blood sample in an 8-color panel using V450, AmCyan, FITC, PE, PerCP-Cy5.5, PE-Cy7, APC and APC-H7 fluorophores. Cytometer Setup and Tracking BD™ Cytometer Setup and Tracking (CS&T) beads provide, in a single vial reagent format, automated determination of BD FACSCanto II configuration baselines and monitoring of performance over time, using BD FACSDiva software. BD™ CompBeads Compensation Particle Sets are designed to optimize compensation settings in multicolor flow cytometry. Particularly helpful when using tandem dye conjugates, these particle sets simplify...

Technical expertise from BD Committed to Customer Success BD Biosciences is fully committed to the success and satisfaction of its customers. Supporting flow cytometry applications for over 35 years, BD training, support, and field service teams are dedicated to helping customers achieve optimal instrument performance, ease of use, and streamlined workflow. With unmatched flow cytometry experience, this world-class service organization is available to help with your BD FACSCanto II product installation, future upgrades, and application support. Training Hands-on training is included with...