A proven research platform for maximum reliability and the highest quality results

A proven platform for maximum reliability and the highest quality results Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high quality results. The BD FACSCanto II provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors. The BD FACSCanto II features many innovations. At the heart of the cytometer, for example, the fluidics system features a fixed alignment flow cell to minimize...

Saves time, improves reproducibility High Performance, Innovative System The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate. A fluidics cart...

Flow cell LED alerts LEDs are located in the front door to monitor each acquisition parameter. Each LED blinks when the signal level reaches a prescribed threshold.

Maximize multicolor performance Innovative Optical Design Delivers Highest Sensitivity The innovative designs for both the excitation and collection optics reduce excitation losses, yielding more information from each sample. The optics of the BD FACSCanto II system consist of an excitation source with up to three lasers, a blue (488-nm, air-cooled, 20-mW solid state), a red (633-nm, 17-mW HeNe) and a violet (405-nm, 30-mW solid state). Laser excitation optics illuminate cells in the sample and collection optics direct light scatter and fluorescence signals through spectral filters to the detectors....

Emission spectra of commonly used fluorochromes Excitation Laser Fluorescence Line (nm) Channel Fluorochromes provided by BD Biosciences BD FACSCanto II Solid State (L1) flow cytometry system The BD FACSCanto II system is designed for these fluorophore combinations. HeNe (L2) Violet (L3)

Productivity support Options and Related Tools to Support Lab Requirements Sample introduction productivity can be gained with the optional BD FACS Loader or the BD High Throughput Sampler (HTS). BD High Throughput Sampler (HTS) Designed for research use, the optional BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition that can speed through a microtiter plate in less than 15 minutes, with less than 1% sample carryover. In high-throughput mode, fast acquisition speed is achieved by synchronizing two high-precision pumps for sample mixing, sample injection, and...

BD FACS Loader The BD FACS Loader is an instrument option that allows walkaway sample introduction to further improve productivity. The BD FACS Loader carousel accommodates up to 40 12 x 75-mm tubes and automatically loads them on the BD FACSCanto II system without operator intervention. Mounted directly on the cytometer, the device includes a drive system, a tube lifter mechanism, and sensors on the sliding drawer. Two sliding doors safely enclose the drawer to protect technicians from moving parts during operation. The BD FACS Loader utilizes compressed air to allow a more reliable tube load...

Versatile, easy-to-use A Powerful Platform for Research Use The BD FACSCanto II system brings BD flow cytometry and multicolor analysis to a new level to help researchers meet complex challenges and advance their research. BD FACSDiva Software for Setup, Acquisition, and Analysis BD FACSDiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the BD FACSCanto II workstation. BD FACSDiva operating software is common across many BD cell analyzers and cell sorters, including BD™ LSR and BD FACSAria™ systems, giving researchers application flexibility. BD...

Lysed whole human blood sample in an 8-color panel using BD Horizon™ V450, AmCyan, FITC, PE, PerCP-Cy5.5, PE-Cy7, APC, and APC-H7 fluorophores. Cytometer Setup and Tracking BD™ Cytometer Setup and Tracking (CS&T) beads provide, in a single vial reagent format, automated determination of BD FACSCanto II configuration baselines and monitoring of performance over time, using BD FACSDiva software. BD™ CompBead Compensation Particle Sets are designed to optimize compensation settings in multicolor flow cytometry. Particularly helpful when using tandem dye conjugates, these particle sets simplify establishing...

Special Order Products for Unique Needs The BD special order research program lets customers leverage the ease of use and proven reliability of the BD FACSCanto II system and configure the instrument with optional PMT filters and lasers to fit precise lab requirements. Choice of Laser Wavelengths The special order BD FACSCanto II research system can be configured from a selection of four laser wavelengths at selectable output power. Designed for research use the special order BD FACSCanto II allows for up to three colors to be configured on each of the available lasers for a maximum of eight...

High Throughput Option The BD FACS Loader and the BD High Throughput Sampler are available for the special order BD FACSCanto II system to meet the needs of high throughput research applications.

Technical expertise from BD BD Biosciences is fully committed to the success and satisfaction of its customers. Supporting flow cytometry applications for over 35 years, BD training, support, and field service teams are dedicated to helping customers achieve optimal instrument performance, ease of use, and streamlined workflow. With unmatched flow cytometry experience, this world-class service organization is available to help with your BD FACSCanto II product installation, future upgrades, and application support. Training Hands-on training is included with each BD FACSCanto II product. Training...