Flow cytometry that adapts to your way of thinking

The BD Influx Cell Sorter Adapts to Your Way of Thinking The BD Influx™ cell sorter is a flexible flow cytometry platform that easily adapts to a researcher’s application or environmental requirements. A modular architecture and a powerful combination of detection capabilities, hands-on controls, and high performance allows researchers to configure the BD Influx system to site and application needs. The BD Influx system was originally designed as a tool for the Human Genome Project. Speed and accuracy continue to be essential requirements. In addition to speed, the platform offers flexibility....

Innovative Fluidics Design Pressurized stainless steel sheath tanks Designed with a Clear Goal: Control Cross Contamination Optimal cellular viability is achieved through a unique nozzle assembly design, and support for aseptic sorting is implemented through a completely exchangeable fluidics path.

Nozzle assembly and exchangeable fluidics Nozzle Assembly and Nozzle Sizes The core of the BD Influx system is the nozzle assembly that features an optimized acoustical coupling, enabling the lowest available stream signal-to-noise droplet formation. This unique design minimizes noise from the droplet formation process and results in high droplet formation rates at relatively low sheath pressures. In addition, the form of the nozzle assembly enables cells to accelerate smoothly to the laser intercept, increasing the viability and functionality of even the most fragile cells. A variety of nozzle...

A Quick and Intuitive Alignment Procedure Intuitive Alignment The optical system that directs lasers to the interrogation point is easy to use, intuitive, and adaptable. Up to five laser paths can be configured and independently aligned. For each laser, light is shaped by individual optics, and final alignment and focusing is achieved via a dedicated lens that steers light to the stream at different angles. Fluorescence is collected through a 20 x 0.6 NA lens and focused on individual mirrored pinholes. This design, combined with a special pinhole camera, allows researchers to image the stream...

Adaptable laser alignment Individual Laser Positioners Independent laser beam alignment and video control of laser intercepts and pinholes allow for fast and intuitive alignment of multiple lasers.

DETECTION OPTIONS Adaptable Detection A Range of Detectors for Application Specific Configurations A range of standard detection modules is available to configure the system based on application needs. Optional, exchangeable detector modules allow for the measurement of small particles, the polarization state of scatter or fluorescence signals, or the spectral properties of a fluorochrome. This flexibility allows the user to adapt the system to the needs of routine or emerging applications. Detection module (4 PMTs) Detection module (3 PMTs) Polarization The BD Influx system can be equipped with...

Chlorella fluorescence intensity (a.u.) Spectral Analyzer Option The BD Influx Spectral Analyzer Option is useful in determining the emission spectrum of a specific fluorochrome to help discriminate homogenous populations. Installed on any photomultiplier port, the analyzer’s spectral grating rotates at a user-defined preset angle while cells pass through the laser. The transmitted signal intensity and grating angle are documented in the list-mode file. The spectral properties of different clusters in the sample are extracted using analytical software. The short interrogation time in a flow cytometer...

Flexible Sorting Unique Sort Modes Allow for Proportional or Positional Sorting The BD Influx system features special sort modes that can be optimized for applications in genomics or proteomics. Proportional sorting allows deflection of cells in proportion to one of the measured signals. For instance, cells can be deflected according to their DNA content to arrange them by the phase in the cell cycle. mRNA hybridization to the stripe of cells of increasing DNA content might serve to study differential gene expression during the cell cycle. Positional sorting positions cells in a 2-dimensional...

Electronics The BD Influx system uses parallel electronics to reach a throughput rate of 200,000 events per second, independent of the number of parameters. Peak and hold circuitry collects the pulses from the photomultipliers, and the peak value is extracted and digitized through a 16-bit ADC. Up to 8 analog signals can be simultaneously processed through an integrator circuitry, extracting area and width measurements. A separate digital subsystem calculates compensation of up to 16 colors in real time and feeds the results back to the system bus. To sort cells, researchers can select subpopulations...

Environmental Control Multiple Levels of Control to Match Application and Environmental Needs The BD Influx aerosol management system draws air away from the operator through a HEPA filter, reducing the amount of aerosols in the sort chamber. Additionally, the BD Influx system can be equipped with a HEPA-filtered enclosure. This customized unit allows researchers to control the HEPA filtered air flow for product and operator safety requirements. The unit's design is optimized to allow it to be adjusted and validated for regional, regulatory, and application-specific requirements. The BD Influx...

Service and Support BD Biosciences instruments and reagents are backed by a world-class service and support organization with unmatched flow cytometry experience. For over 25 years BD has actively worked with researchers to develop tools that help improve workflow, ease of use and performance. With in-depth knowledge and experience, BD delivers comprehensive training, application expertise, excellent technical support and world-class field service. Training Held at BD training centers worldwide, BD Biosciences flow cytometry training courses combine theory and hands-on practice to provide participants...