BD LSRFortessa™ X-20 Cell Analyzer - 2 Pages

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BD LSRFortessa™ X-20 Cell Analyzer

Catalog excerpts

The BD LSRFortessa™ X-20 cell analyzer puts the power of the BD LSR platform into a compact footprint. It can easily fit on the benchtop for more cost-effective space utilization. The instrument delivers the optimal sensitivity and resolution required for multicolor applications. The BD LSRFortessa X-20 cell analyzer can be used to detect up to 18 colors simultaneously and supports up to 5 lasers. In addition to the reduced size, design innovations make filters and detectors more accessible for easier setup of new experiments. Through the BD special order program, customers can choose from 22 different wavelengths and a wide range of power options. This flexibility allows users to configure the instrument to meet their exact requirements for advanced assay development. Optics Excitation Optics Excitation Optical Platform The BD LSRFortessa X-20 optical layout allows for up to five lasers.* Laser Power 355 nm: 15 mW 405 nm: 50 mW 488 nm: 50 mW 561 nm: 50 mW 640 nm: 40 mW Optical Efficiency Power loss at flow cell: <20% of specified laser power Flow Cell Design Rectangular quartz cuvette:Internal cross-section, 430 x180 qm External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light. Fixed optical assembly with spatially separated laser beams. Emission Optics Optical Coupling The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency. Forward Scatter Detection Photodiode detector with a 488/10 bandpass (BP) filter Side Scatter Detector Photomultiplier tube (PMT) with a 488/10 BP filter Emission Optical Design Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD LSRFortessa X-20 uses configurable polygonshaped optical pathways that use signal reflection to maximize signal detection. Please see the filter guide at for information on dye and filter options. Performance Fluorescence Sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE) PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5) APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC) FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A) PE-Cy5 and APC measurements performed using SPHERO™ Ultra Rainbow Calibration Particles (URCP-38-2K) Fluorescence Resolution Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN) Fluorescence Linearity Doublet/singlet ratio of 1.95-2.05 for CEN stained with PI and excited with the 488-nm blue laser Forward and Side Scatter Sensitivity Enables separation of fixed platelets from noise. Forward and Side Scatter Resolution Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes. Side Scatter Resolution Enables separation of 0.5-qm beads from noise. Forward Scatter PMT Option A forward scatter PMT upgrade is available for small particle detection. Requires the 100-mW, 488-nm blue laser. Data Acquisition Rate 40,000 events/sec with beads For Research Use Only. Not for use in diagnostic or therapeutic procedures. Fluidics Sample Flow Rates Front button panel provides three modes: RUN, STANDBY, and PRIME Continuously adjustable flow rate, plus three preset flow rates: LO: 12 qL/min MED: 35 qL/min HI: 60 qL/min Standard Fluidic Reservoirs One 8-L sheath container and one 10-L waste container provided. Recommended Fluidics Option BD FACSFlow™ supply system: automated fluidics system, which includes a rolling cart and two 20-L Cubitainer® packages

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Software: BD FACSDiva™ v8.0 or later Workstation1 Operating System Microsoft® Windows® 7 Professional 32-bit OS SP1 Processor Intel® Xeon® E3-1240v3 3.4 GHz CPU RAM 4 GB Options Monitor Options Two 19-in. LCDs, 2560 x 1024 resolution (standard) One 23-in. LCD, 1680 x 1050 resolution (optional) One 24-in. LCD, 1920 x 1200 resolution (optional) Printer Options Options vary by location. Please check with your local sales representative. High Throughput Option The BD™ High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well...

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