BD Rhapsody™ HT Single-Cell Analysis System
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BD Rhapsody HT Single-Cell Analysis System ™ Fast track single-cell research without compromise

Increase throughput. Decrease time to answer. No compromise. Single-cell analysis technologies are important tools utilized to understand the intricacies and heterogeneity of cellular populations and cellular systems. Cell-specific information gathered from these tools that are lacking in traditional genome-wide techniques allows deeper insights from hundreds of thousands of cells at single-cell resolution. The BD Rhapsody™ HT Single-Cell Analysis System allows flexible sample processing and cell capture from hundreds to hundreds of thousands of single-cells using a gentle and robust...

BD Rhapsody™ System quality, higher throughput Flexible cartridge design Subsample beads • Creates flexibility with experimental design • Partial use of cartridge enables: • Tool to measure sample quality – Running more or different types of experiments – Processing samples together or on different days • Share beads across sites with collaborators Archive beads Low multiplet rate per lane • Equivalent data obtained from fresh beads and stored beads • 2.5% @ 10,000 cell load • 5.2% @ 25,000 cell load • Support flexible and collaborative workflow approach • Backup for underperformed or...

High cell capture and low multiplet rate across cell inputs Desired number of cells Live cells loaded** Viable cells captured in well with a bead Capture rate Cell multiplets *Mix of PBMC, Jurkat, Ramos and THP1 cells **BD Rhapsody™ Scanner hemocytometer count Microwell Barcoded bead Viable cell Figure 1. Four cell types (PBMCs, Jurkat, Ramos and THP1) were pooled and loaded in duplicate at 10,000, 25,000 or 55,000 cells per lane on an 8-lane cartridge. Cell capture rates were high and multiplet rates were low at all cell load concentrations. The BD Rhapsody™ Scanner provides a measure of...

Confidence in every experiment with the BD Rhapsody™ Scanner visual workflow QC The BD Rhapsody™ Scanner can be used to provide quality control measures at different stages of the workflow by direct imaging through an intuitive user interface for a multi-sample workflow. Experimental setup – Enter sample and experiment information to track samples through single-cell workflow Scan and status – Watch in real time the status of the lanes being scanned Multi-sample selection – Allow users to select and name up to eight lanes for processing through the cartridge scan workflow Image analysis –...

A complete single-cell multiomics solution Cell staining (optional) Cell capture High-dimensional data analysis BD Rhapsody™ SingleCell Multiplexing Kit BD Rhapsody™ HT Xpress System BD Rhapsody™ NGS Library Prep Kits Single-cell capture, bead-based mRNA isolation and bead retrieval BD Rhapsody™ Whole Transcriptome Analysis Amplification Kit BD Rhapsody™ HT Single-Cell Analysis System BD Rhapsody™ Targeted mRNA Kit and AbSeq Amplification Kit Single-cell capture, beadbased mRNA isolation, bead retrieval with imaging and cell workflow QC BD Rhapsody™ TCR/BCR Amplification Kit BD® AbSeq...

Ordering information BD Rhapsody™ HT Single-Cell Analysis System Description BD Rhapsody™ HT Xpress Package* Reagents BD Rhapsody™ 8-Lane Cartridge BD Rhapsody™ Enhanced Cartridge Reagent Kit BD Rhapsody™ Targeted mRNA & AbSeq Amplification Kit BD Rhapsody™ TCR/BCR Amplification Kit, Human BD Rhapsody™ TCR/BCR Amplification Kit, Mouse Companion products BD® AbSeq Single-Vial Reagents Contact for more info BD AbSeq Immune Discovery Panel BD Immune Response Panel BD Human Single-Cell Multiplexing Kit BD® Mouse Single-Cell Multiplexing Kit BD® Flex Single-Cell Multiplexing Kit A BD® Flex...

For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD Life Sciences, San Jose, CA 95131, USA bdbiosciences.com BD, the BD Logo and BD Rhapsody are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. © 2023 BD. All rights reserved. BD-80577 (v1.0) 0323