BD Rhapsody™ Immune Response Targeted Panel
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BD Rhapsody Immune Response Targeted Panel (Human) ™ Targeted human immune gene panel for the BD Rhapsody Single-Cell Analysis System Validated panel focuses on genes relevant to phenotyping human immune cells Targeted sequencing approach requires dramatically fewer reads than a 3’ whole transcriptome sequencing approach Reproducible assay empowers numerous applications The human immune system is an intricate defense network of organs and cell types that protect against pathogens, environmental antigens and cancer. The immune response consists of complex interactions among several cell types. These cells are involved in surveillance and recognition of foreign pathogens or cells, secretion and recruitment of inflammatory signals, coordination and expansion of specific adaptive immune cells, and maturation of adaptive cells for immune memory and antibody production. Understanding how these processes function normally and in instances of dysregulation are fundamental to understanding such diseases as inflammation, infection and cancer. Universal oligo bead Univ = Universal sequence UMI = Unique molecular index CL = Cell label dT = oligo(dT) Universal oligo Univ BD Rhapsody Immune Response PCR 1 primer BD Rhapsody Immune Response PCR 2 primer Library forward primer Univ Final amplification Library reverse primer Sequencing adapter Univ Expertly designed panels give you the right genes Drawing from BD’s 40 years of experience in immunology, the panel content was chosen to comprise relevant phenotypic markers, including those routinely used in FACS analysis. Panel genes include those involved in cytokine and chemokine secretion, interleukin signaling and transcription factors involved in cell differentiation and maturation. Panel content was specifically developed to amplify transcripts most relevant to immune-cell function, rather than constitutively expressed genes (e.g. ribosomal genes). This focused approach to sequencing dramatically improves assay efficiency compared to a whole transcriptome assay. Targeted approaches save both sequencing costs and analysis time, allowing researchers to better utilize resources for other immune studies. Tailored libraries for more targeted sequencing The BD Rhapsody ™ Immune Response Panel (Human) is an assay for single-cell research of the human immune system with the BD Rhapsody Single-Cell Analysis System. The panel allows for more cost efficient cellular phenotyping of single cells. Researchers interested in immunology, translational research, or drug development gain a powerful tool to identify, explore and understand human immune cell function. Sequencing adapter & library index Figure 1. cDNA synthesis and library construction chemistry using the BD Rhapsody Immune Response Targeted Panel (Human). The BD Rhapsody Immune Response Targeted Panel (Human) utilizes multiplex PCR for detecting 399 genes chosen for profiling human immune cells. After cells are lysed in the BD Rhapsody Cartridge, beads containing captur

magnetically retrieved for cDNA synthesis. Included primers are used for several rounds of gene-specific nested PCR for library construction. The final PCR amplification products for sequencing contains sequencing adapters, a cell label, unique molecular index (UMI) and up to 400 bp of the 3’ end of the target gene. Assay products can be sequenced on Illumina MiSeq, NextSeq, HiSeq2500 and HiSeq4000 sequencers. Table 2: BD Rhapsody Immune Response Targeted Panel (Human) metrics Your target genes, delivered your way Total reads recommended per cell For cell type identification For direct...