BD Rhapsody Single-Cell Analysis System and BD Rhapsody Express Single-Cell Analysis System TM

A robust, high-throughput microwell based single-cell partitioning system for reliability in single-cell workflows The BD Rhapsody™ Single-Cell Analysis System allows high-throughput capture of multiomic information from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of nextgeneration sequencing (NGS) libraries. NGS libraries are sequenced and analyzed to provide high-dimensional resolution of single cells. Trust the BD Rhapsody™ System with your precious samples Microwell technology • No...

Simple, streamlined workflow for success with your experiments Prepare Single-Cell Suspensions Prime and Treat Cartridge Prepare Single-Cell Suspensions • Cells can be stained with the BD® Single-Cell Multiplexing Kit and/or BD® AbSeq Ab-Oligos Prime and Treat Cartridge • Automated pipettes for fluidic exchanges Single-Cell Capture Workflow Single-Cell Capture Workflow 1 Load Cells 1a Cell Load Scan Load Beads Load Cells Load 575 µL cell suspension Clogging of channels are not a concern as there are no microfluidic channels Gentle settling of cells by gravity allows capture of fragile cells The...

High cell capture and low multiplet rate across cell inputs Live cells loaded** Viable cells captured in well with a bead Capture rate *Mix of PBMC, Jurkat, Ramos and THP1 cells Desired number of cells ** BD Rhapsody™ Scanner hemocytometer count Figure 2. A mix of PBMCs, Jurkat, Ramos and THP1 cells were loaded at 10,000, 20,000 or 40,000 cells per cartridge Cell capture rates were high and multiplet rates were low at all cell load concentrations. Results may vary based on cell type and isolation method. Theoretical (Poisson) Cell types with disparate size and morphology recovered in similar...

Confidence in every experiment with visual workflow QC The BD Rhapsody ™ Scanner can be used to provide quality control measures at different stages of the workflow, such as cell capture rate and cell multiplet rate, by direct imaging. Scanner metrics can confirm quality of input cell sample and success of each step of the cartridge workflow; assess viability of cells until cells are lysed; and provide a reliable estimate of the number of cells retrieved by sequencing. This information gives users the power to change course and troubleshoot experiments, if necessary, before expensive downstream...

A complete single-cell multiomics solution from your trusted partner in flow cytometry From reagents required for cell multiplexing and staining to assays and bioinformatics tools, streamlined protocols and technical support, BD provides the tools you need to accelerate your discoveries using single-cell multiomic analysis. Sample Collection and Preservation BD Vacutainer® Tubes for cell and biomarker preservation Cell Staining Data Analysis Large portfolio of high-quality reagents including pre-titrated, high quality BD ® AbSeq Antibody-Oligo Conjugates and BD ® Single-Cell Multiplexing Kits...

A continuous ecosystem for high-dimensional biology research BD Biosciences flow cytometry instruments have enabled researchers to successfully conduct flow cytometry analysis for over 45 years. The BD Rhapsody ™ System extends this capability and facilitates the analysis of thousands of genes and proteins simultaneously at the single-cell level. Characterize cell populations with a high-parameter flow cytometry panel on a BD cell analyzer, transfer the same panel to a BD cell sorter for isolation of cell populations of interest, and perform further in-depth multiomic analysis of sorted cells...

BD flow cytometers are Class I Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD Life Sciences, San Jose, CA, 95131, USA bdbiosciences.com BD, the BD Logo, Horizon, Rhapsody, SeqGeq and Vacutainer are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. © 2021 BD. All rights reserved. BD-28693 (v1.0) 0321