Another Innovative Test Designed by WANTAI WANTAI SARS-CoV-2-IGRA Diagnostic Kit for T Cell activity to SARS-CoV-2 For monitoring immune response after vaccination y = 167.64x0.9554 R2 = 0.9984 Leading infectious diseases diagnostic test manufacturer Your Global Partner in IVD IGRA measure a person's immune reactivity to SARS-CoV-2. T lymphocytes from most persons that have been infected with SARS-CoV-2 will release interferon gamma (IFN-γ) when mixed with antigens derived from SARS-CoV-2. Wantai Interferon Gamma Release Assay (IGRA) is whole-blood test that can aid in diagnosing SARS-CoV-2 infection and monitoring of the immune response after vaccination. E-mail: [email protected] Website: www.ystwt.cn

Procedures Quantitative detection of Interferon Gamma (IFN-γ) A: Sample Preparation Dispense into culture tubes 1 Collect whole blood samples Each well contains anti-IFN-γ antibody Coated Microplates Add 20μl of sample dilution buffer, 50μl of samples and 50μl of standards incubate at 37°C for 15 minutes avoiding light Coloring: Add 50μl of Solution A and 50μl of Solution B Wash plate: 5 times Read the absorbance value: read the absorbance value at 450nm/ 600~650nm Stopping Reaction: Add 50μl of Stop Solution Double wave length Performance Analysis A group of 129 vaccinated individuals were tested...