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Enzyme Histochemistry kits and solutions

Enzyme Histochemistry kits and solutions

Enzyme Histochemistry kits and solutions

Product catalog summary
Overview
Microscopic examination of muscle biopsies is crucial for diagnosing neuromuscular disorders. Laboratories face challenges such as reagent toxicity, solution standardization, storage requirements, and result reproducibility. Bio-Optica offers ready-to-use enzyme histochemistry kits to address these issues, ensuring safer and more consistent results.
Enzyme Histochemistry Kits
  • Acid Phosphatase (30-30118LY): Highlights enzymatic activity in macrophages and lysosomes, useful for identifying necrosis and regeneration.
  • Alkaline Phosphatase Stain (30-30121LY): Evaluates enzymatic activity to highlight phagocytosis and inflammation sites in muscle biopsies.
  • NADH Diaphorase (30-30113LY): Distinguishes between type 1 and type 2 muscle fibers, often used with ATPase evaluation.
  • Esterase (30-30122LY): Highlights enzymatic activity in denervated fibers.
  • Phosphorylase (30-30123LY): Highlights enzymes involved in glycogenolysis.
  • ATPase (30-30125LY): Determines muscle fiber types, used with cryostat sections of striated muscle.
  • Cytochrome C Oxidase (30-30115LY): Evaluates enzymatic activity.
  • Myoadenylate Deaminase (30-30116LY): Highlights enzymatic activity of AMPDA.
  • Phosphofructokinase (30-30117LY): Determines if glycogen storage disease is due to phosphofructokinase deficiency.
  • Succinic Dehydrogenase (30-30114LY): Evaluates enzymatic activity in mitochondria.
Fixatives and Solutions
  • Fixatives: Includes Backer fixative (30-30111) and Fixative for Acid Phosphatase (30-30120).
  • Staining Solutions: Methyl Green Buffered Solution (30-30119), Oil Red O Solution (30-30112), and Trichrome Stain Gomori (30-30110).
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Catalog excerpts

Enzyme Histochemistry kits and solutions-1

Improving Pathology ENZYME HISTOCHEMISTRY Kits and solutions Microscopic examination of sections of muscle biopsies is an essential tool in the diagnosis of neuromuscular disorders. Any laboratory choosing to conduct histo-enzymatic tests on muscle biopsies encounters a series of problems: •high toxicity of certain reagent •solutions that are difficult and complex to standardize •storage of solutions at -20° •poor reproducibility of final results. To overcome all these problems, Bio-Optica has developed ready-to-use kits for enzyme histochemistry. Enzyme histochemistry kits eliminate the difficulties and risks associated with the preparation of stain solutions, thus ensuring reproducible re

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Enzyme Histochemistry kits and solutions-2

Acid phosphatase Alkaline phosphatase Stain 30-30118LY 10 ml Highlighting enzymatic activity of acid phosphatase. Present in macrophages and lysosomes; identifies necrosis and regeneration. 30-30121LY 10 ml Evaluating enzymatic activity of alkaline phosphatase. Useful for highlighting the sites of phagocytosis and inflammation in muscle biopsies. NADH diaphorase 30-30113LY 10 ml Evaluation of NADH diaphorase activity. Staining useful for distinguishing type 1 and type 2 muscle fibers, often used in conjunction with ATPase evaluation. 30-30123LY 10 ml Highlighting enzymes belonging to the phosphorylase...

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