Atlas ClearSight Tablets with TAE Cat. No. BA50203 BAS0039 Pack Size 100 x 0.5 g 2 x 0.5 g SAMPLE Storage & Shipping: Store at RT, protected from light, shipping at room temperature. Applications: • Ideal for routine DNA and RNA gel electrophoresis and blotting assays • Used dye is non-carcinogenic alternative to Ethidium bromide. Description: Atlas ClearSight Tablets contain everything necessary for an easy preparation of an agarose gel in desired gel percentage. Atlas ClearSight Tablets are packed in a convenient blister pack. Atlas ClearSight Tablets with TAE contain: • Agarose • TAE powder • Atlas ClearSight DNA stain Only RT or cooler pure water is needed to add for preparation of an agarose gel. Do NOT use hot water for dissolving the tablet Do NOT add any buffer This composition is optimized to yield high resolution of sharp DNA bands with high sensitivity and low background. Atlas ClearSight DNA Stain emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (~270 nm; ~290 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is centered at ~530 nm. Thus, Atlas ClearSight DNA Stain is compatible with a wide variety of gel reading instruments. Protocol: • Use the bottle or flask that is at least 3 times of the volume of the solution being prepared. • Add an appropriate number of agarose tablets in the water and do NOT add any buffer! See the table below to achieve needed gel percentage. Gel % 1% 1.5% 2% • • • • • • • 1 tablet 32.5 ml water 21.5 ml water 16.25 ml water 2 tablets 65 ml water 43 ml water 32.5 ml water Soak the tablet in the pure water for 3-5 minutes (or until it is dissolved) before heating. For tablet dissolving use water which is at room temperature, DO NOT use hot water. Heat the solution until it is clear and visually all the particles are dissolved. Cool the gel to 60-70ºC and cast the gel, into the gel tray. The thickness of gel should be<0.5cm. Run the gel in TAE running buffer. Detect the bands under Blue light or UV illuminator. Bioatlas Riia 181a, 50411, Tartu, Estonia, tel: +372 55624867 e-mail: info@bioa

Safety warnings and precautions: Caution when using hot, viscous solutions! Use suitable safety gear and open bottle gently to avoid accidents. Atlas ClearSight DNA Stain is non-carcinogenic and according to the Ames test it causes significantly fewer mutations than Ethidium bromide. Atlas ClearSight DNA Stain is non-carcinogenic but may irritate skin and eyes. This product is designed for research purposes and in vitro use only. According to common laboratory safety practice, it is recommended to wear protective clothing, gloves and safety glasses. Please refer to www.bioatlas.com for Material...