Bio 30+ Amino Acid Analyzer System The gold standard dedicated Amino Acid Analyzer for physiological fluids Powered by Harvard Bioscience, Inc. Powered by Harvard Bioscience, Inc.

Amino acid analysis in physiological fluids “A relatively busy laboratory, we typically run approximately 1,000+ physiological samples. We have been very pleased with the performance of our Bio 30+ since installation over a decade ago and highly recommend it for the any analytical laboratory environment.” - Laboratory Manage

Investigating metabolic disorders is a crucial and complex task. With over 42 significant amino acids and their derivatives implicated in the accurate analysis of Inborn Errors of Metabolism1, including Phenylketonuria (PKU), laboratories need an accurate and reliable analysis system that delivers unequivocal results quickly. This compact instrument allows users to accurately detect and quantify amino acids and their derivatives in complex physiological samples. A powerful tool... • Accurate…Exceptional analysis quality with low-matrix interference • P recise... Unequivocal peak identification...

Bio 30+ System The gold standard in amino acid analysis Bio 30+ Amino Acid Analysis System • Biochrom quality parts used cooled autosampler. It is designed support team. Our applications team • Rapid service from our to operate 24/7 and ideal for busy clinical and research labs. This compact instrument can handle plasma, serum, urine, dried blood spots and CSF with low matrix interference and without costly and time-consuming re-equilibration. dedicated technical and engineering can help with full screening methods or specific short methods from our application database built over many years....

Key Features • B io 30+ Analyzer System with air-cooled autosampler • Choice of column (with top-up resin) • Starter pack of ready-to-use reagents • Spare parts and consumables kit • HP computer and monitor • B iosys software and OpenLab EZChrom Edition data handling software • On-site customer training A chromatographic system specifically for amino acids The Bio 30+ System is a cation exchange chromatography system coupled with a highly specific detection system using post column derivatisation with ninhydrin reagent. Amino acids are separated according to their net charge determined by the...

Robust and stable chemistry Biochrom’s patented EZ Nin solution is a unique form of Ninhydrin which needs no preparation or special conditions to remain stable long term, meaning less time to set up, and no more wasting the last few mL in the bottle. Chemicals and consumables are available either as complete kits or as individual buffers to enable continuity of analysis. All reagents are stable at room temperature and guaranteed to give accurate and reproducible results with a 3-year shelf life. On the instrument, buffers and reagents are stored under an inert gas to ensure stability. Choice...

Free Amino Acid Analysis in Physiological Samples The Bio 30+ System enables you to recognize a characteristic amino acid chromatographic profile to aid in free amino acid analysis. The quantification of the amino acids may can assist in investigating metabolic abnormalities. For reference purposes, chromatograms of reference material, normal plasma and urine samples are shown. Physiological Standard (Part Number 80-6002-80) analyzed on the Bio 30+ High Performance method

Plasma Analysis A full screening is achievable in less than two hours (injection to injection) in physiological samples with the Accelerated System. Rare key markers are clearly separated as shown in the plasma sample below. Plasma sample containing unusual amino acids. 570nm and 440nm channel. Urine Analysis Urine sample analyzed on the Bio 30+ High Performance System.

Analysis of Tyrosine and Phenylalanine: The phenylalanine short method allows a fast and accurate separation of tyrosine, phenylalanine and homocystine within a short single run. Samples can be analyzed within 30 minutes, with an injection to injection repeat time of 50 minutes. Amino acid reference material analyzed on a Bio 30+ equipped with the High Performance column. Norleucine used as the internal standard. Branched Chain Amino Acids Analysis: Using the Bio 30+ System, the levels of the branched chain amino acids leucine, isoleucine, and valine, can be determined accurately, using norleucine...

Total Homocysteine Assay Elevated plasma total homocysteine has emerged as an important risk factor in the development of vascular disease3. The Bio 30+ System allows a rapid analysis of homocysteine levels in plasma with an analysis time of 24min injection to injection. Physiological standard spiked with homocysteine. Norvaline is used as the internal standard. Sulfocysteine Using the Bio 30+ System, a short program for the analysis of s-sulfocysteine has been developed that allow its separation from phosphoserine. Analysis of sulfocysteine in urine sample

Bio 30+ System Technical Specifications Reproducibility Area: better than 1.5% RSD at 10 nanomoles. Retention time: better than 0.5% RSD. 9 - 15 pmoles (primary amino acids detection at 570nm) Time of analysis (based on the separation of 45 physiological amino acids): Accelerated analysis: 115 min injection to injection High Performance analysis: 170 min injection to injection Analytical Column High pressure PEEK column packed with Ultropac 8 cation exchange resin. Peltier heating/cooling system. Eluent System 6 buffer system (5+1 regeneration solution) stored on the instrument at room temperature...

**Please Note: Use of non-Biochrom approved columns, reagents, buffers, etc. may invalidate the warranty of the Biochrom Amino Acid Analyzer. 1 Differential diagnosis of (inherited) amino acid metabolism or transport disorders. W. Blom & J.G.M.Huijmans, Amino Acids (1992) 2:25-67 2 Vademecum Metabolicum Manual of Paediatrics. J. Zschocke & G. Hoffmann, 2ed.Milupa GmbH 2004 3 Rapid Analysis of Homocysteine Levels. A.Lolia & S. Bee, For more than 50 years, Harvard Bioscience’s division Biochrom has been delivering the industry’s leading Gold Standard Amino Acid Analysis (AAA) instruments to life...