CoolCell Alcohol Free Cell Freezing Containers
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Alcohol-Free Cell Freezing CoolCell alcohol-free cell freezing containers ensure standardized controlled-rate -1oC/minute cell freezing in a -80oC freezer - without alcohol or any fluids. Proven for use with a variety of cell types including stem cells, primary cells and cell lines. The patent-pending CoolCell technology utilizes a thermo-conductive alloy and highly-insulative outer materials to control the rate of heat removal and make cell cryopreservation reproducible. CoolCell units are easy to use; simply fill with cryogenic vials and place in a -80oC freezer. CoolCell Container Alcohol-Filled Container • Consistent -1oC/minute freezing rate No alcohol • No fluids • No pre-cooling required Requires 250ml alcohol • Replace alcohol every 5 uses • Keep track of number of uses • Pre-cool alcohol in refrigerator Inconsistent freeze rate • Alcohol degradation induces variability • Two circles of wells – different freeze rates for each Approximately $350/year • Change alcohol weekly • Disposal of hazardous waste Difficult to handle • Screw cap very difficult to remove when frozen • Frozen unit is slippery and cold to touch • No variability from freeze to freeze, more consistent, faster results • Higher cell viability • No maintenance, no waste • No hidden cost • Easy to use • Minimal impact on freezer and frozen contents No variability • All vials have uniform freeze rate • Radially symmetric design ensures consistency No on-going cost • No alcohol purchase or disposal No stuck lids • Lid comes off easily when frozen • Not cold to the touch when removing from the -80oC freezer Quick re-use time • Ready to use again after five minutes Low impact on freezer • 1/3 the heat impact on freezer compared to alcohol-filled units Wait between runs • Takes >1 hour for device to return to room temperature for re-use Large thermal mass impacts local freezer area • Large heat capacity removed from alcohol impacts nearby samples CoolCell Consistency “We run a registry in which large amounts of Peripheral Blood Mononuclear Cells are processed for long-term cryopreservation. After testing the CoolCell, we found slightly better cell viability (>90%) than with our current cell freezing containers, and there is no isopropanol waste generated. Overall, the CoolCell has set a new bar in cryopreservation.” - Rohit G., Stanford University Cryopreservation and Thawing of Cells Yokoyama WM, Thompson ML, and Ehrhardt RO Current Protocols in Immunology, Nov 2012 Five consecutive freeze cycles show highly reproducible freezing profiles.

CoolCell Performance Stem Cells Human Embryonic Stem Preadipccytes Breast Cancer Stem Colon Cancer Stem Gliablastoma Cancer Stem Mouse Embryonic Stem Human Endothelial Progenitor Fig 1. HESC - RC10. After 2 weeks in LN2, 3 vials from each method were thawed and cells counted immediately (Day 1) and after 3 days growth (Day 3). CoolCell method showed 33% increase in cells after 3 days growth. Fig 2. Alamar Blue reduction assay for proliferation analysis of RC10 cells. Cells frozen in CoolCell grew more quickly leading to higher proliferation rates and more cells. Primary Cells Neonatal...

CoolCell LX Cell Freezing Container CoolCell FTS30 High-Capacity Cell Freezing Container For 12 x 1 mL or 2 mL cryogenic vials, 1 mL fill per vial. Radially-symmetric for uniform vial freezing. Numbered wells for easy sample identification. Beveled lid for secure gripping and easy opening. Exposed vial tops when lid is open for quick, organized removal of frozen samples. For 30 x 1 mL or 2 mL cryogenic vials, 1 mL fill volume per vial. Controlled micro-convection for uniform freezing of 30 vials. Removable cryogenic vial tray for one-step transfer of samples into and out of freezing...

Cell Cryopreservation Accessories CoolCell Protecting Agents Add your serum, media or buffers to these single-use cell biology grade DMSO or glycerol cryoprotectants. Eliminates wasting large bottles of DMSO and difficult pipetting of viscous glycerol. Dual graduations percentage and mL - on the back for measuring. Prepare from 5% (50 mL) to 20% (12.5 mL) of cryopreservation solution. Item No. BCS-3010 BCS-3011 Description CoolCell Protecting Agent, DMSO CoolCell Protecting Agent, Glycerol CoolCell Filler Vial To ensure cell freezing rate consistency and uniform results, insert a CoolCell...