Standardized Cell Freezing • High post-thaw viability and proliferation • Highly reproducible freeze runs • -1˚C/minute freeze rate in _ -80oC freezer • Numbered vial holes for identification • Beveled design for quick _ tube retrieval • No alcohol No on-going cost No maintenance CoolCell LX freezing containers provide a highly reproducible -1oC/minute freezing rate for cell cryopreservation providing more cells, faster, for your research. No alcohol is required; simply place CoolCell LX filled with cryogenic vials in a -80oC freezer. The new beveled design allows easy opening with access to the vial tops, simplifying transfer. Numbered vial holes allows indexing and quick removal of cryogenic vials. CoolCell LX accommodates 12 cryogenic vials and is available in two sizes for small 1 to 2ml cryogenic vials or large 3.5 to 5ml cryogenic vials. Item No. BCS-405 BCS-405G BCS-405O BCS-405PK Description ● CoolCell LX, purple ● CoolCell LX, green ● CoolCell LX, orange ● CoolCell LX, pink CoolCell is proven for use with a variety of cells, including: The solid state core and micro-convection technology precisely balance heat removal during freezing period to ensure repeatable, consistent cooling all the way to cryogenic storage temperature. Stem Cell/ Pre-Stem Cell Human Embryonic Stem Preadipccytes Breast Cancer Stem Colon Cancer Stem Gliablastoma Cancer Stem Mouse Embryonic Stem Human Endothelial Progenitor Primary Cells Neonatal Keratinocytes Human WBCs Mouse WBCs Human CD34+ Muscle Human Tendon Fibroblasts Melanoma Tumor Human Cardiac VentricularHuman Cardiac Atrial Cell Lines CHO LnCap HTB77 A549 HeLa

Stem Cells Primary Cells Cell Lines Fig. 1. Human embryonic stem cells, RC-10 were frozen using the technique indicated, thawed after 2 weeks in LN2, and counted immediately (Day 1) or after 3 days of growth (Day 3). Fig. 2. Alamar blue reduction assay for proliferation assessment showed cells frozen in CoolCell grew more quickly, leading to more total cells. Fig. 3. PBMC cells frozen in CoolCell or “Mr Frosty” isoproponal containers were thawed and live cell counts obtained the trypan blue method. CoolCell performed as well as Mr. Frosty without the requirement for alcohol. Fig. 4 HUVEC cells...