dsDNA Quantification Broad Range Kit (For use with microplate readers) Catalog No. 40540B: 1000 assays Storage: at 4°C and room temperature upon receive Description The dsDNA Quantification Broad Range Kit (For use with microplate reader) is developed for double stranded DNA quantification. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and DNA Standards. Simply dilute the BR Dye with the BR Dilution Buffer, add DNA sample, then read the concentration using microplate reader. The assay is accurate in the linear range from 4 to 1000 ng, and is highly selective for double-stranded DNA over RNA (Figure 1). Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assay (Table 1). Table 1. Effect of contaminants in the dsDNA BR Assay Final concentration Concentration in Contaminant Result in the assay 10-μL sample Sodium chloride 10 mM 200 mM OK Magnesium chloride 2 mM 40 mM OK Sodium acetate 10 mM 200 mM OK Ethanol 1% 20% OK Phenol 0.10% 2% OK SDS 0.001% 0.02% OK Triton X-100 0.001% 0.02% OK dNTP 100 μM 2 mM OK BSA 20 μg/mL 400 μg/mL OK RNA 0.5X 0.5X OK ssDNA 0.5X 0.5X OK

BioDynami Component • dsDNA • dsDNA Storage Condition • dsDNA BR Dye (protect from light): store at 4°C, stable for 6 months. • dsDNA BR Standards: store at 4°C, stable for 6 months. • dsDNA BR Dilution Buffer: store at room temperature, stable for 6 months. Reagent & Equipment Needed (not provided in this kit) • Plastic container (disposable) for diluting the BR Dye with the BR Dilution Buffer • Microplates for fluorescence-based assays \ Assay Parameters • Assay temperature: The assays are designed to be performed at room temperature (22-28°C). Store the Dilution Buffer at room temperature....

Quality Control Kit components passed stringent functional quality test. Product Use Limitation This product is developed and sold for research purposes and in vitro use only. Please refer to BioDynami.com for Material Safety Data Sheet of the product. Limited Label License The product is developed and sold exclusively for research purposes and in vitro use only. The product or its any individual component has not been tested for use in diagnostics or drug development, and is not suitable for administration to human or animal. The purchaser of this product is granted a limited, non-transferable...