Genomic DNA Extraction Kit (HMW, Magnetic Beads)
Genomic DNA Extraction Kit (HMW, Magnetic Beads)
- HMW Genomic DNA size: 50 kb to 150 kb.
- High purity achieved through magnetic beads technology.
- No need for centrifuge, vacuum, or column during the extraction process.
- Sample Types: Cells, Blood, and Tissues.
- Sample Amounts: Up to 0.5 million cells, 200 µl whole blood, or 10 mg tissue.
- Genomic DNA Yield: 3-6 µg for cells, 4-12 µg for blood, up to 30 µg for tissues.
- Elution Volume: 100 µl for all sample types.
- Optical Density (O.D.) Ratios: 260/280 between 1.8-2.0, 260/230 above 2.0.
- BioDynami's kit offers fast processing times: 30 minutes for cells, 50 minutes for blood, and 90 minutes for tissues.
- Compared to other vendors like Circulomics, Revolugen, and Qiagen, BioDynami's kit is noted for its simplicity, high yield, and high purity.
- Other methods like Phenol/Chloroform extraction are time-consuming and involve hazardous reagents.
- Genomic DNA Extraction Kit for Cells (Cat.# 50014).
- Genomic DNA Extraction Kit for Blood (Cat.# 50015).
- Genomic DNA Extraction Kit for Tissues (Cat.# 50016).
Catalog excerpts
HMW: 50 kb to 150 kbHigh Purity with Magnetic Beads Technology The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using magnetic beads. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity. 50 kb to 150 kb High Purity Simple • No centrifuge A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells
Open the catalog to page 1
Tissue Samples Qiagen 3.5 3.5 4 Long workflow; Genomic Tips hours hours hours Not very high molecular weight. Phenol/Chloroform >6 >6 >6 Long workflow; Hazardous reagents; Extraction hours hours hours DNA resuspension is difficult. *Workflow times are estimated based on protocol and internal testing **Based on internal testing More details at https://biodynami.com/product/genomic-dna-extraction-hmw-magnetic-beads/
Open the catalog to page 2All BioDynami catalogs and technical brochures
NGS Library Preparation1 Page
DNA Ladder1 Page
Magnetic Beads4 Pages
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