Methylation Specific Bisulfite-Seq (MSBS)
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Methylation Specific Bisulfite-Seq (MSBS)
Product catalog summary
Overview: The document provides information about BioDynami's Methylation Specific Bisulfite Sequencing (MSBS) technology, which is designed for the enrichment of methylated CpG sites, offering single base resolution and cost savings.
Technologies Compared: The document compares MSBS with other sequencing technologies such as Whole Genome Bisulfite Sequencing (WGBS), Reduced Representation Bisulfite Sequencing (RRBS), and Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq). Key features include:
- Enrichment of Methylated CpG Sites: MSBS and MeDIP-Seq focus on methylated regions, while WGBS sequences the entire genome.
- Single Base Resolution: Achieved by MSBS, WGBS, and RRBS, but not by MeDIP-Seq.
- Cost Efficiency: MSBS, RRBS, and MeDIP-Seq are noted for being cost-effective compared to WGBS.
- Whole Methylated CpG Coverage: MSBS and WGBS provide comprehensive coverage, whereas RRBS covers only about 10%.
Product Offerings: BioDynami offers several library prep kits for MSBS, each with different primer options:
- Methylation Specific Bisulfite-Seq Library Prep Kit (with non-index primers) - Cat.# 30101
- Methylation Specific Bisulfite-Seq Library Prep Kit (with index primers) - Cat.# 30102
- Methylation Specific Bisulfite-Seq Library Prep Kit (with unique dual index primers) - Cat.# 30103
Examples: The document mentions examples of high and low methylated regions in the human genome to illustrate the application of the technology.
Contact Information: For more details, visit the website biodynami.com or contact via phone at (256) 872-0805 or email at [email protected].
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Catalog excerpts
Enrichment of methylated CpG sites CpG Enrichment Simple workflow Two reaction steps Single Base Resolution Hands-on time Walk-away time Cost Saving Fast Total time: • No library loss by bisulfite conversion • No expensive methylated adaptor MSBS (BioDynami) Email: [email protected] I Fast Simple Affordable High-Quality
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Product Name More details at biodvnami.com/product/methvlation-specific-bisulfite-sea/ Email: [email protected]
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