Catalog excerpts
A ll the way with animal component-free solutions Animal ComponentFree (ACF) Recombinant Trypsin Solutions A lternative to porcine/bovine trypsin • Animal Component-Free (ACF) E liminates the risk of viruses or other potential adventitious agents found in animal derived components. Pure enzyme solutions increase specificity and eliminate contaminating activities found in lower purity enzymes. Free of chymotrypsin, carboxypeptidase-A and other protease contaminants. Prevents the toxic effects induced by non-desirable proteases. • High Activity Maximizes the yield of functionally viable cells. Recombinant Trypsin-EDTA Solution (Cat. No. 03-079-1) accelerates the dissociation phase. Results in efficient dissociation of adherent cell types (including primary and sensitive cells) from surfaces and tissues. Optimized for hMSCs, from a variety of sources, cultured in both serum-free and serum–containing systems. • Enzyme Inhibition I nactivation with Soybean Trypsin Inhibitor (
Open the catalog to page 1Comparison of hMSC Dissociation with Various Trypsin Solutions R ecovery of hMSC- Adipose Tissue (AT) cultured in MSC NutriStem XF medium after dissociation with three different dissociation solutions F igure 1. hMSC-AT, 3 days post split I – Cells were equally seeded (5000cells/cm2) in MSC NutriStem® XF medium. The dissociation procedure was carried out at 37° using: A . Recombinant Trypsin Solution, without EDTA (BI’s Cat. No. 03-078-1) B . Crystalline Trypsin (BI’s Cat. No. 03-047-1) (high purity) C . Trypsin sol. C (BI’s Cat. No. 03-053-1) (crude trypsin) R apid...
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