A ‫ ‏‬ll the way with animal component-free solutions Animal ComponentFree (ACF) Recombinant Trypsin Solutions A ‫ ‏‬lternative to porcine/bovine trypsin • Animal Component-Free (ACF) E ‫ ‏‬liminates the risk of viruses or other potential adventitious agents found in animal derived components. ‫ ‏‬Pure enzyme solutions increase specificity and eliminate contaminating activities found in lower purity enzymes. ‫ ‏‬Free of chymotrypsin, carboxypeptidase-A and other protease contaminants. ‫ ‏‬Prevents the toxic effects induced by non-desirable proteases. • High Activity ‫ ‏‬Maximizes the yield of functionally viable cells. ‫ ‏‬Recombinant Trypsin-EDTA Solution (Cat. No. 03-079-1) accelerates the dissociation phase. ‫ ‏‬Results in efficient dissociation of adherent cell types (including primary and sensitive cells) from surfaces and tissues. ‫ ‏‬Optimized for hMSCs, from a variety of sources, cultured in both serum-free and serum–containing systems. • Enzyme Inhibition I ‫ ‏‬nactivation with Soybean Trypsin Inhibitor (

Comparison of hMSC Dissociation with Various Trypsin Solutions R ‫ ‏‬ecovery of hMSC- Adipose Tissue (AT) cultured in MSC NutriStem XF medium after dissociation with three different dissociation solutions F ‫ ‏‬igure 1. hMSC-AT, 3 days post split I – Cells were equally seeded (5000cells/cm2) in MSC NutriStem® XF medium. The dissociation procedure was carried out at 37° using: A ‫ . ‏‬Recombinant Trypsin Solution, without EDTA (BI’s Cat. No. 03-078-1) B ‫ . ‏‬Crystalline Trypsin (BI’s Cat. No. 03-047-1) (high purity) C ‫ . ‏‬Trypsin sol. C (BI’s Cat. No. 03-053-1) (crude trypsin) R ‫ ‏‬apid and...