Catalog excerpts
NutriStem® MSC Culture System A complete xeno-free, serum-free system for the growth and expansion of hMSCs • Defined, xeno-free, serum-free medium Redefining stem cell excellence and advancing clinical applications • Superior proliferation of hMSCs Defined, serum-free, xeno-free reagents are essential tools for all human mesenchymal stem cell (hMSC) research having potential clinical applications. The NutriStem® MSC Culture System includes defined reagents ideal for translational research use. hMSCs cultured in serum-free, xeno-free NutriStem® MSC XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles and karyotypic stability over long-term culture. • Supports long-term growth and differentiation potential • FDA Drug Master File Cell Number Growth Curve of hMSC-BM NutriStem® MSC XF Medium is designed for optimal growth and expansion of hMSCs derived from a variety of sources, including bone marrow (BM-hMSC), adipose tissue (AT-hMSC), Wharton’s jelly (WJ-hMSC), placental tissue (PT-MSC), and umbilical cord matrix (UC-hMSC). Commercial SF medium Serum-containing medium Long Term Growth of hMSC-AT Serum-containing medium (Weiss) NutriStem® MSC XF medium STEMPRO® MSC SFM (Invitrogen) MesenCult™ - XF (SCT) Passage Number Days Post Seeding Figure 1: NutriStem® MSC XF Medium promotes superior proliferation and expansion of hMSCs over time as compared to other serum-free and serum-containing media. Commercial serum-containing medi
Open the catalog to page 1• Xeno-free, purified human fibronectin (hFN) • Optimized for serum-free cultures • For hMSC proliferation and differentiation • Ready-to-use, defined • Recombinant trypsin solutions • Chemically defined, animal component-free, protein-free Recombinant Trypsin Solution Figure 2: MSC Dissociation Solutions. Recovery of BM-hMSC after dissociation with either Recombinant Trypsin Solution or the common Trypsin EDTA Solution (porcine) and re-seeding on plates pre-coated with the MSC Attachment Solution and cultured in NutriStem® MSC XF Medium. Images were taken on Day 5 postdissociation (100X)....
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