DANGERS OF CONTAMINATION IN CELL CULTURE LABS DIFFERENT MYCOPLASMA SPECIES HAVE BEEN IDENTIFIED TODAY, AT LEAST 20 DISTINCT SPECIES OF MYCOPLASMA HAVE BEEN ISOLATED FROM CONTAMINATED CELL CULTURES OF CELL CULTURE CONTAMINATION IS MADE UP OF ONLY 6 SPECIES M. orale OM. hyorhinis OM. arginini M. fermentans OM. hominis OA. laidlawii DID YOU KNOW? THE MOST COMMON MYCOPLASMAS THAT CONTAMINATE CELL CULTURES ORIGINATE FROM HUMAN, BOVINE, AND SWINE MYCOPLASMA CELLS CAN ATTACH TO AN INFECTED CELL AT ANY GIVEN TIME EACH MYCOPLASMA CELL CAN GROW TO CFU’S PER ML OF CONTAMINATED MEDIA IN ONLY (CFU = colony forming unit) CD D0 YOU TEST FOR MYCOPLASMA? « rO/ vi?:. 35% m. * x acell csns™ OF CELLS IN CELL BANKSARE '$«${ V# f 35% SNEEZING of LAB TECHNICIANS are carriers of mycoplasma TALKING OTHER SOURCES OF CONTAMINATION: • Liquid nitrogen tanks • Other contaminated cultures • Used pipettes TOP 5 REASONS TO TEST: 1 LOSS OF TIME AND MONEY 2 INVALID SCIENTIFIC DATA 3 MISLEADING OR RETRACTED PUBLICATION 4 LOSS OF CELL LINES 5 CONTAMINATION BETWEEN LABS ROUTINELY TEST EVERY 2 WEEKS to 3 MONTHS DEPENDING ON CELLS IN THE LAB ALWAYS PRACTICE GOOD STERILE TECHNIQUE © EXAMINE AND SCREEN YOUR CELLS OFTEN (£) AVOID TALKING OVER YOUR CELLS QUARANTINE ALL NEW CELL CULTURES AND ANIMAL PRODUCTS ENTERING LAB RELIABLE COST-EFFECTIVE EFFICIENT This infographic has been produced solely for informational purposes only. Please note that data has been extracted from various publications and sources, and not all contextual evidence may be represented. Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443982/, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584481/, http://www.nature.com/nprot/journal/v5/n5/full/nprot.2010.43.html, http://en.wikipedia.org/wiki/Mycoplasma, http://csmedia2.corning.com/LifeSciences/Media/pdf/cccontamination.pdf: http://www.intechopen.com/books/biomedical-tissue-culture/contamination-of-tissue-cultures-by-mycoplasmas © 2016 Biological Industries USA. All Rights Reserved.