Next-generation chemically defined serumfree, animal component free medium designed to support the growth of Vero cells

Isolated from the kidney of the African green monkey by Yasumura and Kawakita in Japan (1962), the Vero cell line is used for various purposes, most importantly for the production of cell culture-based viral vaccines. Reasons for the extensive use of the Vero cell line are the consistent high viral yields and relatively easy adaptation for growth in bioreactors on microcarriers, thus allowing greater vaccine purity and as well as quantity1. The Vero cell line is among a very limited list of cell lines that have been approved by health authorities worldwide for the production of human vaccines...

• Suitable for 2D and 3D cultures • Chemically defined - no plant or animal components • Very low protein concentration • Lot-to-lot consistency • Reduced risk of contamination • Optimal for the production of various viruses (incl. measles, enterovirus, polio) • Suitable for direct adaptation • Easy downstream product purification • Produced in a cGMP-compliant facility in a xeno-free dedicated production line. Product Name Cat. No. Size Storage From small scale research to large scale production Minimize regulatory concerns Animal-derived components (e.g. fetal bovine serum and chicken embryos)...

Technical Data Vero cell growth and cell density in a 2D culture system Defined NutriVero™ Flex 10 was tested in a defined animalcomponent free system for cell growth and cell density. Utilizing a 2D culture system, NutriVero™ Flex 10 defined medium showed equivalent performance as undefined extracts containing medium (VP-SFM) (figure 1). Figure 1: Vero cells were seeded in T25 flasks at a cell density of 40,000 cells/cm2 and incubated at 37ºC in a humidified atmosphere and 5% CO2 with NutriVero™ Flex 10 or VP-SFM. At indicated times post seeding cells were harvested and re-suspended in medium...

Virus production in 2D and 3D systems Virus production assessment in a 2D culture system Figure 4: Vero cells were seeded in 6-well plates at a cell density of 30,000 cells/cm2 and infected with various viruses. Following 7 days of culture, the cultures showed positive Cytopathic Effect (CPE), and the supernatant was harvested and analyzed for virus titer. 8 Viable isolated cells (x106) For initial assessment of defined NutriVero™ Flex 10 viral production capacity, Vero cells were seeded in a 2D culture system and infected with various viruses (Figure 4). Virus titer for NutriVero™ Flex 10 was...

Insignificant effect of soy hydrolysate on cell growth and virus titer Vero cell growth in 2D culture system with the addition of plant hydrolysate 3.0 2.5 Generations To assess the effect of plant hydrolysate on the performance of defined NutriVero™ Flex 10, 0.1% soy hydrolysate was added to the medium. Cell concentration was measured upon seeding and during harvesting of 3 passages and the number of generations was calculated. The addition of hydrolysate had no effect on cell growth in 2D culture system. Figure 7: Vero cells were seeded in T25 flasks at a cell density of 40,000 cells/cm2 and...

Summary Chemically defined, serum-free, animal-component-free NutriVero™ Flex 10 medium demonstrates excellent results in Vero cell growth and virus yield in both 2D and 3D culture systems. Containing solely recombinant components and no plant extracts (no hydrolystates) NutriVero™ Flex 10 shows equal performance and in some cases is superior to undefined competitor medium. Furthermore, the addition of plant hydrolysate to NutriVero™ Flex 10 did not enhance cell growth and virus yield. Utilizing NutriVero™ Flex 10 removes variability that is in correlation with undefined extracts thus reducing...

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