DNA/RNA Amplification
91Pages

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Catalog excerpts

DNA/RNA Amplification - 1

DNA/RNA Amplification DNA Amplification RNA Amplification Real-Time PCR Customized PCR

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DNA/RNA Amplification - 2

Overview AccuPower® PreMix series is an innovative PCR master mix product range designed to make your PCR, reverse transcription RT-PCR and qPCR experiments easier and more cost effective than ever. With a wide range of applications and unparalleled ease of use and stability, AccuPower® PCR PreMix series will be an important addition to your collection of research tools. Each tube or plate you order has all the components of a PCR master mix -including enzyme, buffer and dNTPs - lyophilized with a patented stabilizer that maintains full activity of reagents for over one month at room...

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Selection Guide – By Application ■ RNA Amplification cDNA Synthesis Kits Applications AccuPower® RocketScriptTM Cycle RT AccuPower® RocketScriptTM RT (RNase H-) One-step RT-PCR Kits Applications Standard RTPCR RT-PCR of Secondary Structured RNA High Specificity & High Sensitivity AccuPower® RT-PCR AccuPower® Dual-HotStartTM RT-PCR AccuPower® Dual-HotStartTM RT-PCR (with UDG) AccuPower® RocketPlex RT-PCR Multiplex RT-PCR AccuPower® RocketScriptTM RT-PCR Prevent Carryover Contamination ■ Real-Time PCR Applications Products HotStart qPCR AccuPower® GreenStarTM qPCR AccuPower® DualStarTM qPCR...

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DNA/RNA Amplification - 4

Specifications of AccuPower® Premix Series ■ PCR & One-step RT-PCR Product

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DNA/RNA Amplification - 5

Selection Guide of Enzymes Product

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DNA/RNA Amplification - 6

DNA Amplification Selection Guide ········································································································································3 Standard PCR Kit AccuPower® PCR PreMix ················································································································································7 AccuPower® PCR PreMix (with UDG)························································································································· 9 AccuPower® Taq PCR...

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AccuPower® PCR PreMix For Standard PCR, Dried-type Premix with Top DNA Polymerase ● Reproducibility: AccuPower® PCR PreMix is manufactured under strict ISO 9001 quality control conditions to ensure reproducible PCR performance. ■ Specifications ● Enzyme: Top DNA polymerase ● 5’ to 3’ exonuclease activity: No ● 3’ to 5’ exonuclease activity: No ● 3’ - A overhang: Yes ● Fragment size: ■ Description AccuPower® PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA polymerase, dNTPs, reaction buffer, tracking dye, and our patented stabilizer. AccuPower® PCR PreMix includes our...

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DNA/RNA Amplification - 9

■ Description Polymerase chain reaction (PCR) is a powerful technique that can amplify a single molecule of DNA to levels detectable on an agarose gel. Therefore, a presence of a small amount of DNA in the reaction can amplify and lead to a false positive result. Such effect is common, where there may be products from previous PCR amplifications that can carryover to the next PCR cycle (termed: carry-over contamination). To avoid this, the most common way is to avoid carry-overcontamination is touse dUTP as a substitute for dTTP for all PCR performed in a lab and treating all PCR reactions...

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