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DNA/RNA Amplification

DNA/RNA Amplification
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DNA/RNA Amplification

Product catalog summary
Overview
The document provides comprehensive information on Bioneer's AccuPower® PreMix series, which includes PCR, RT-PCR, and qPCR products designed to enhance experimental efficiency and reliability. These products are lyophilized with a patented stabilizer, ensuring long-term stability at various temperatures.
Specifications
The AccuPower® PreMix series offers a range of products tailored for DNA and RNA amplification, real-time PCR, and one-step RT-PCR. Each product is designed for specific applications such as standard PCR, HotStart PCR, high fidelity PCR, and multiplex PCR. The series includes options with or without loading dye for direct gel loading.
Product Features
  • Stability: Enhanced enzyme stability at high temperatures, extending enzyme half-life and improving yield.
  • Ease-of-use: Pre-dispensed reactions in tubes/wells, requiring only the addition of primers and template.
  • Sensitivity and Reproducibility: Demonstrated superior sensitivity and strict quality control for reproducibility.
Applications
  • DNA Amplification: Standard PCR, HotStart PCR, gene cloning, TA cloning, high fidelity PCR, long-range PCR, GC-rich PCR, and multiplex PCR.
  • RNA Amplification: Standard RT, high-efficiency RT, RT of secondary structured RNA, and HotStart RT.
  • Real-Time PCR: HotStart RT, HotStart qPCR, qPCR with intercalating dye or TaqMan probe, and prevention of carryover contamination.
Ordering Information
AccuPower® PCR PreMix is available in various formats, including thin-wall microtubes, 8-tube strips, and 96 or 384-well plates, with different reaction volumes to suit experimental needs.
Experimental Data
Figures in the document compare the sensitivity and specificity of Bioneer's PCR PreMix products with competitors, demonstrating superior performance in various conditions.
Transport and Storage
  • Transport Temperature: Room temperature
  • Storage Temperature: -20°C
Additional Products
The document also details various DNA polymerases and RT-PCR kits, including CycleScript, RocketScript, and their RNase H Minus variants, highlighting their features, benefits, and applications.
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Catalog excerpts

DNA/RNA Amplification-1

DNA/RNA Amplification DNA Amplification RNA Amplification Real-Time PCR Customized PCR

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DNA/RNA Amplification-2

Overview AccuPower® PreMix series is an innovative PCR master mix product range designed to make your PCR, reverse transcription RT-PCR and qPCR experiments easier and more cost effective than ever. With a wide range of applications and unparalleled ease of use and stability, AccuPower® PCR PreMix series will be an important addition to your collection of research tools. Each tube or plate you order has all the components of a PCR master mix -including enzyme, buffer and dNTPs - lyophilized with a patented stabilizer that maintains full activity of reagents for over one month at room temperature,...

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DNA/RNA Amplification-3

Selection Guide – By Application ■ RNA Amplification cDNA Synthesis Kits Applications AccuPower® RocketScriptTM Cycle RT AccuPower® RocketScriptTM RT (RNase H-) One-step RT-PCR Kits Applications Standard RTPCR RT-PCR of Secondary Structured RNA High Specificity & High Sensitivity AccuPower® RT-PCR AccuPower® Dual-HotStartTM RT-PCR AccuPower® Dual-HotStartTM RT-PCR (with UDG) AccuPower® RocketPlex RT-PCR Multiplex RT-PCR AccuPower® RocketScriptTM RT-PCR Prevent Carryover Contamination ■ Real-Time PCR Applications Products HotStart qPCR AccuPower® GreenStarTM qPCR AccuPower® DualStarTM qPCR AccuPower®...

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DNA/RNA Amplification-4

Specifications of AccuPower® Premix Series ■ PCR & One-step RT-PCR Product

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DNA/RNA Amplification-5

Selection Guide of Enzymes Product

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DNA/RNA Amplification-6

DNA Amplification Selection Guide ········································································································································3 Standard PCR Kit AccuPower® PCR PreMix ················································································································································7 AccuPower® PCR PreMix (with UDG)························································································································· 9 AccuPower® Taq PCR PreMix·····································································································································...

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DNA/RNA Amplification-7

AccuPower® PCR PreMix For Standard PCR, Dried-type Premix with Top DNA Polymerase ● Reproducibility: AccuPower® PCR PreMix is manufactured under strict ISO 9001 quality control conditions to ensure reproducible PCR performance. ■ Specifications ● Enzyme: Top DNA polymerase ● 5’ to 3’ exonuclease activity: No ● 3’ to 5’ exonuclease activity: No ● 3’ - A overhang: Yes ● Fragment size: ■ Description AccuPower® PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA polymerase, dNTPs, reaction buffer, tracking dye, and our patented stabilizer. AccuPower® PCR PreMix includes our super-processive...

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