DNA/RNA Oligonucleotide Synthesis Oligonucleotide - AccuOligo® QC & Order system Oligonucleotide FAQs User Protocol

DNA/RNA Oligonucleotide Synthesis Oligonucleotide - AccuOligo® ································································································································3 Standard Oligonucleotide ···········································································································································5 HT-Oligo™ (High Throughput Oligonucleotide) ································································································7 Modified Oligonucleotide ···········································································································································8...

Oligonucleotide [AccuOligo®) Overview High quality oligos and reasonable price. Founded in 1992, Bioneer is one of leading suppliers of synthetic oligonucleotides in the world (DNA and RNA). In-house production of raw materials (phosphoramidites, reagents & solvents) along with automated oligo synthesis and purification systems result in superior quality oligonucleotides at a reasonable price. Since Bioneer manufactures all the components of our oligonucleotides, we manage the quality control every step of the way - ensuring that you receive only the highest quality product. Bioneer's oligonucleotides...

Oligonucleotide (AccuOligo®) Bio-RP purification: Phosphoramidite, which serves as the coupling monomer in oligonucleotide synthesis, has a DMT-protected 5’-OH group to prevent repetitive coupling reaction during the individual addition steps of synthesis. Only the n-mer oligos with the expected length, not n-1, n-2, n-3 … mer oligos, have the 5’-DMT group provided DMT was not removed after final round of synthesis (AKA “Trityl-On” mode). Because of this, Bioneer has developed unique Bio-RP resin, which attracts the hydrophobic DMT group, as well as an automated column purification process. In...

Recommended Purification Methods by Application Synthesis Scale (Guaranteed quantity of starting materials) ■ Description Standard oligonucleotide synthesis service includes synthesis of up to 130 mer oligonucleotides. A variety of synthesis scales, as well as a choice of purification methods, (Bio-RP, HPLC or PAGE), depending on your application, can be selected. ■ Features and Benefits • Free Bio-RP purification: Near HPLC purity at a standard oligo price • Quick turnaround time: 24 - 48 hr from order to shipping in most cases • Broad range of modifications available: Nearly 300 modifications;...