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DNA/RNA Purification

DNA/RNA Purification
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DNA/RNA Purification

Product catalog summary
Overview of Nucleic Acid Extraction Kits
This document provides a comprehensive overview of nucleic acid extraction kits by Bioneer, emphasizing their applications in molecular biology, biotechnology, and laboratory medicine. The quality of extracted nucleic acids is crucial for experiments such as PCR, sequencing, and molecular diagnostics.
Types of Extraction Kits
  • ExiPrep™ DNA/RNA Extraction Kit: An automated system for extracting high-quality nucleic acids from up to 16 samples using a pre-loaded buffer cartridge system and silica magnetic particles.
  • MagListo™ Magnetic Nanobead-based Extraction Kit: Utilizes magnetic nanobeads for quick and efficient nucleic acid separation, adaptable for various scales.
  • AccuPrep® DNA/RNA Extraction Kit: A manual kit using a single spin column with glass microfiber filters for high-purity and high-yield DNA/RNA extraction.
  • AccuZol™ Products: Employ traditional organic solvent methods for RNA extraction, suitable for large input volumes or manual workflows.
Specific Kits and Their Features
  • Blood Genomic DNA Kit: Extracts genomic DNA from blood samples with high yield and purity using automated instruments.
  • Tissue Genomic DNA Kit: Designed for extracting DNA from animal tissues, featuring a Tissue Lysis Buffer with Proteinase K.
  • Bacteria Genomic DNA Kit: Suitable for Gram (-) and Gram (+) bacteria, providing an optimized buffer for cell lysis and resuspension.
  • Plant Genomic DNA Kit: Extracts DNA from plant samples using a Plant Lysis Buffer.
  • Tissue Total RNA Kit: Extracts total RNA from animal tissues, optimized for various tissue types.
  • Plant Total RNA Kit: Extracts RNA from plant samples using a Plant Lysis Buffer.
Applications and Experimental Data
The kits are applicable in PCR, Real-Time PCR, gene cloning, SNP analysis, and more. Experimental data, including agarose gel electrophoresis and Real-Time PCR results, demonstrate the kits' effectiveness in extracting high-quality nucleic acids.
Viral DNA/RNA Kit
  • Description: Designed for extracting viral DNA/RNA from various cell-free body fluids and swabs using ExiPrep™ 16 series and ExiProgen™ instruments.
  • Features and Benefits: Pre-dispensed reagents, processes up to 16 samples per run, uses patented magnetic silica beads.
  • Applications: Suitable for PCR, RT-PCR, Real-Time PCR, and RT-qPCR.
  • Experimental Data: Real-Time PCR results demonstrate effectiveness in extracting viral DNA from serum.
MagListo™ 5M Kits
  • Cell Total RNA Extraction Kit: Rapid extraction of total RNA from various cell types using Magnetic Nanobeads, requiring only eight minutes for mini-scale extraction.
  • Tissue Total RNA Extraction Kit: Quick RNA extraction from tissues and cells using Magnetic Nanobeads, with a 10-minute extraction time for mini-scale.
  • Genomic DNA Extraction Kit: Rapid genomic DNA extraction from various samples using Magnetic Nanobeads, with a five-minute extraction time for mini-scale.
  • Plant Genomic DNA Extraction Kit: Rapid genomic DNA extraction from plant samples using Magnetic Nanobeads.
  • Forensic Sample DNA Extraction Kit: Rapid DNA extraction from forensic samples using Magnetic Nanobeads.
AccuPrep® Kits
  • Nano-Plus Plasmid Extraction Kits: Extract highly-purified plasmid DNA from cultured bacterial cells using a modified alkaline lysis protocol.
  • Genomic DNA Extraction Kit: Rapid extraction of genomic DNA from blood, tissue, and cultured cells without phenol/chloroform extraction.
  • GMO DNA Extraction Kit: Extracts DNA from agricultural products and processed foods for GMO detection.
  • Stool DNA Extraction Kit: Extracts DNA from stool samples, eliminating contaminants and enzyme inhibitors.
  • Viral RNA Extraction Kit: Extracts viral RNA from cell-free samples for various RNA viruses.
  • PCR and Gel Purification Kits: Purify DNA fragments from PCR products and agarose gels.
Experimental Data and Ordering Information
The document includes experimental data demonstrating reduced preparation times and successful restriction enzyme digestion tests, comparing AccuPrep® kits with competitors. Each kit is listed with its catalog number and description, providing options for different reaction sizes.
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Catalog excerpts

DNA/RNA Purification-1

DNA/RNA Purification Automatic Nucleic Acid Extraction: ExiPrep™ Magnetic Bead Type Manual Nucleic Acid Extraction: MagListo™ Spin Column Type Manual Nucleic Acid Extraction: AccuPrep® 96-Well Vacuum Block Type Manual Nucleic Acid Extraction Solution Type Manual Nucleic Acid Extraction: AccuZoE™ Accessory

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DNA/RNA Purification-2

Automatic Nucleic Acid Extraction: ExiPrep™ DNA/RNA Purification·····························································································································3 Genomic DNA Extraction Kit Tissue Genomic DNA Kit·················································································································································6 Bacteria Genomic DNA Kit·············································································································································8 Plant Genomic DNA Kit·······································································································································...

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DNA/RNA Purification-3

DNA/RNA Purification Overview The extraction and purification of nucleic acid is very important step in molecular biology, biotechnology, biochemistry, laboratory medicine, etc, because the result of experiments, such as PCR/RTPCR, Real-Time PCR/RT-qPCR, sequencing, subcloning, gene cloning, molecular diagnostics, etc, depends on the quality of an extracted and purified nucleic acid. In addition, it is important to select the extraction kit optimized for samples in order to extract DNA/RNA effectively from blood, animal tissue, culture cell, plant tissue as well as biological specimen. Bioneer...

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DNA/RNA Purification-4

Blood Genomic DNA Kit ■ Specifications Starting volume Elution volume Expected yield ■ Application PCR, Real-Time PCR, Cloning, SNP analysis, Pharmacogenomics, and Genetics ■ Experimental Data ■ Description Blood Genomic DNA Kit enables extraction of genomic DNA from whole blood samples with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 series (ExiPrep™ 16 Plus, ExiPrep™ 16 Dx) and ExiProgen™. ■ Features and Benefits ● All reagents are pre-dispensed into a pre-filled buffer cartridge The kit contains all required consumables such as disposable filter tips,...

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