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Gene Synthesis

Product catalog summary

Gene Synthesis Service
Gene synthesis is a cost-effective method for enhancing gene research, providing 100% sequencing confirmation. Bioneer offers services such as sequence design, codon optimization, synthesis, cloning, and sequencing. This service is useful for antibody construction, organism production optimization, gene construction, and protein modification. The procedure includes order submission, sequence analysis, synthesis, cloning, and product shipment. Pricing starts at $0.39 per base pair, with additional charges for complex sequences.

Mutagenesis Service
This service constructs mutant genes for protein research, offering site-directed mutations with 100% sequence accuracy. Pricing is based on mutation type and complexity. The procedure includes order submission, sequence analysis, synthesis, and product shipment. Additional services include high-yield plasmid DNA production.

Codon Library Mutagenesis Service
Bioneer provides codon-based mutagenesis to create gene libraries tailored to specific species, enhancing protein expression and function. Applications include protein modification and antibody construction. Pricing is based on mutation sites, with discounts for bulk orders. The procedure involves order submission, sequence analysis, and product shipment.

Gene Cloning Service
Details on the Gene Cloning Service were not fully provided in the document excerpt.

Overview: Bioneer offers comprehensive gene cloning and synthesis services, utilizing advanced molecular biology techniques and quality control to ensure high-quality results. These services support various applications in synthetic biology, including protein modification, antibody construction, and organism production optimization.

Features and Benefits:

100% Sequence Guarantee: All custom clones are confirmed by sequencing to ensure accuracy.
Value Pricing: Competitive pricing for research needs, with automatic DNA sequencing included.

Applications:

Protein Modification: Codon optimization enhances protein expression efficiency, and mutant libraries can yield proteins with improved functions.
Antibody Construction: Custom antibodies can be optimized for expression in host organisms, with libraries available for screening efficient variants.
Organism Production Optimization: Gene expression optimization maximizes industrial biological production efficiency.

Procedure:

Order Submission
Sequence Analysis/Input Synthesis Information
Synthesis Process Confirmation
Cloning/Sequencing
Product Shipment

Ordering Information:

Gene Cloning Service: Pricing starts at $200.00 per cloning, with additional costs for cultured cells and larger inserts.
Service Period: Varies based on vector and insert size, ranging from 5 to 15 working days.
Additional Services: High-yield plasmid DNA production and gene synthesis discounts available.

Vector Information: Customers can select from various vectors, including high copy, low copy, in vitro transcription, and expression vectors, each with specific features and antibiotic markers.

AccuGeneBlock Service:

Description: Provides synthesis of double-stranded DNA fragments (<1 kb) in as little as 3 days.
Features: Fast and accurate synthesis, value pricing, and free codon optimization.
Applications: Suitable for large gene synthesis, protein modification, antibody gene construction, and organism production optimization.

FAQs:

Gene Synthesis: Bioneer offers a range of services including gene synthesis, mutagenesis, and cloning, with applications in antibody construction and protein modification.
Ordering Process: Customers can obtain quotations and place orders via email, with technical support available for assistance.
Gene Storage: Information is stored for six months post-delivery, with options for immediate disposal upon request.

Change in Gene Sequence: Customers can modify their gene sequence before order completion. A fee of $50 applies for changes less than 9 bp at either end, and $100 for changes less than 20 bp inside the gene. Immediate contact is advised for sequence changes.

Order Cancellation Fees: Cancelling within 5 days of order confirmation incurs a 50% charge of the quoted price. After 5 days, the charge increases to 80%.

Mutagenesis & Gene Cloning Service Requirements: Customers must provide a cloning vector (150-200 ng/µl, >10 µl) and DNA templates (50 ng/µl, >10 µl) via FedEx to the specified address in South Korea. If a vector is unavailable, Bioneer can procure one, adding its cost to the final quote.

Increasing Vector Quantity: An additional $100 and five days are required for each 100 µg increase of vector with the cloned gene.

AccuGeneBlock Service Limitations: DNA synthesis is limited to 100 bp - 1 kb without high/low GC content, sequence repeats, or inverted sequences. For adverse factors, the Gene Synthesis Service is recommended.

Toxic Sequence Synthesis: Toxic sequences can be synthesized with a consent form from an institute or government, ensuring use for research purposes only.

User Protocol for Synthetic Gene:

To increase DNA: Add 20 µl of DW or TE buffer, prepare competent cells, transfer plasmid DNA, incubate, and isolate using Accuprep® kit.
For experiments: Add buffer, measure DNA, and proceed with restriction enzyme cut or PCR.

Delivery is in lyophilized form, recommended storage at -20°C after dissolving.

Catalog excerpts

Gene Synthesis Gene Synthesis Service Mutagenesis Service Codon Library Mutagenesis Service Gene Cloning Service Vector Information AccuGeneBlock Service Gene Synthesis FAQs User Protocol

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Gene Synthesis Gene Synthesis Service············································································································· 3 Mutagenesis Service·················································································································· 5 Codon Library Mutagenesis Service ··················································································· 7 Gene Cloning Service················································································································ 9 Vector Information ······················································...

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Gene Synthesis Service Overview Gene synthesis is the most cost-effective way to enhance your gene research. You can have your gene cloned in your hand with 100% sequencing confirmation data within for most cases. You can save your time and money through Bioneer’s Gene Synthesis Service than through conventional way to clone a gene. If you like, our free codon optimization will increase protein expression rates and can enhance protein function. In addition, we can construct virtually any targets of DNA easily. Bioneer also offers Mutagenesis as well as Cloning Services. Bioneer strives to provide...

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■ Ordering Information Gene Synthesis Service S Additional charges will apply for gene segments containing complexities such as high or low GC content, repeat sequences or homopolymeric runs. If any of your sequence is found to contain the above listed complexities, you will be contacted by our Gene Synthesis Specialist. S When choosing the subcloning service with a commercial vector, a vector purchase cost will be billed separately. S Synthesized genes will be sequenced. Only 100% sequence identity product will be shipped. S If it's founded as gene which is toxic or inhibit the growth in E....

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■ Description The fastest and most cost effective way to construct a mutant gene for protein structure and functional research or to increase enzyme function is to use our Mutagenesis Service combined with our Gene Synthesis Service. Site-directed mutation such as Point Mutation as well as deletion, insertion mutation services are available. For unsurpassed quality and value pricing, partner with Bioneer today. ■ Features and Benefits • 100% sequence guarantee: Individual synthetic genes are confirmed by automatic DNA sequencer (ABI 3730) • Value pricing: The bestvaluefor your research dollar...

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Mutagenesis Service ● Quotation and Ordering 1. Download the ordering form(s) from the link on web site (www.bioneer.co.kr). 2. Fill out the form and email the form to: [email protected] 3. We will review the order and send you service information (final quote and service period) via email. 4. If you decide to go ahead with the service, please confirm the service by an email ([email protected]). 5. Service will be initiated when we receive the raw material from you 50% charge of service bill for cancellation in 5 days. 80% charge of service bill for cancellation after 5 days. Free...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.

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