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BQCKit Company is one of the three differentiated business lines of Bioquochem S.L. We are a Spanish biotechnology company specialized in the design, development and manufacture of biological kits for the measurement of biological parameters. These kits present characteristics of specificity and are geared especially for fields of health, food and cosmetic. All BQCkit Assay Kits are designed, developed and manufactured in Spain by BQCkit team, which is formed by PhDs and researchers from the University of Oviedo with several years of experience. The company strengths are innovation, personalized...

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ICON KEY Available sizes Volume of sample required Types of sample compatible Linear range of the assay Type of detection Time of reaction

DMPD ANTIOXIDANT CAPACITY ASSAY KIT This kit is recommended for antioxidant capacity measurements in foods, beverages and biological samples. It measures the antioxidant activity of compounds that are able to transfer hydrogen atoms. In our assay a solution of DMPD in the presence of a suitable oxidant solution, can form a stable and colored radical cation which shows an absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce a discolorations of the solution which is proportional to their amount. DMPD (uncolored) + Oxidant...

FRAP ANTIOXIDANT CAPACITY ASSAY KIT KF01003 The kit is designed for the antioxidant capacity measurement in biological samples. BQCkit FRAP assay kit is recommended for total antioxidant activity of single antioxidants in aqueous solution and added to plasma. The assay described here measures the ferric reducing ability of plasma (FRAP). At low pH, when a ferric complex is reduced to the ferrous form (Fe2+), an intense blue color with an absorption maximum at 593 nm develops. CHARACTERISTICS 500 tests 10 pL Biological fluids ORAC ANTIOXIDANT CAPACITY ASSAY KIT KF01004 The kit is designed...

LIPID PEROXIDATION ASSAY KIT KB03002 The kit is designed to determine lipid peroxidation in biological fluids. This measures the MDA and HNE concentrations as an index of lipid peroxidation. Reactions between indoles and aldehydes (MDA and HNE) gives a diindolylalkane (chromophore) whose maximal absorbance is in the 580-620 nm region. Reagent A + MDA ^ C (Amax = 586 nm) Reagent A + HNE ^ C (Amax = 586 nm) CHARACTERISTICS 200 tests 100 pL Any samples containing lipid hydroperoxides THIOL QUANTIFICATION ASSAY KIT KB03007 The kit is designed to measure free thiols and disulfides in biological...

PROTEIN CARBONYLATION ASSAY KIT The kit is designed for the measurement of oxidized proteins in many kind of samples. Protein carbonylation occurs providing an integrated assessment of oxidative damage. The most employed method for evaluation of the content of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). This molecule reacts with carbonyl groups leading to the formation of the stable 2,4-dinitrophenylhydrazone. The dinitrophenyl group (DNP) can be detected and quantified spectrophotometrically at 365–375 nm. 2,4-dinitrophenylhydrazine (DNPH)+ Carbonyl  Imine (hydrazone)...

NITRITE/NITRATE DETERMINATION ASSAY KIT Nitrate/Nitrite Determination Kit is recommended for the determination of nitrite and nitrate, which is a method for the detection of nitric oxide formation. The nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors, to eliminate interferences. The detection is based on the final product detection (diazonium compound, λmax= 540 nm) obtained after nitrite reaction in several steps with sulfanilamide. NO3 -  NO2 – (Nitrate Reductase) Sulfanilamide + Nitrite   Diazonium product (λMax = 543 nm) Standard and plate included Very...

The kit is designed to measure superoxide dismutase (SOD) activity in biological samples. SOD Activity Assay Kit (Colorimetric) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Xanthine + O2  Uric Acid + H2O2 + 2O2- (Xanthine Oxidase) 2O2- + Tetrazolium Salt (WST-1)  WST-1 Formazan (colored, λMax = 450 nm ) Standard...

BRADFORD PROTEIN ASSAY KIT KB03003 The kit is designed for protein quantification in biological samples. The method is based on an absorbance shift of the dye Coomasie Brilliant Blue in which under acidic conditions the red form of the dye is converted into its blue form to bind to the protein being assayed. The formation of the dye-protein complex takes about two minutes and remains stable for an hour, so the procedure is very fast and the time for trial is not limiting. Protein (basic and aromatic side chains) + Coomasie G-250 ^ Protein- Dye Complex ( XMax = 595 nm ) *_j Plasma, serum, urine,...

BCA PROTEIN ASSAY KIT KB03005 The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. The principle of the BCA assay relies on the formation of a Cu2+ protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu+. The purple colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex displays a strong absorbance at 562 nm. Protein (Cysteine, tyrosine, tryptophan amino acid residues) + Cu...

ECL WESTERN BLOTTING SUBSTRATE! KB03020 The kit is designed for protein quantification in biological samples. BQCkit ECL Western Blotting Substrate Kit detects proteins which are bond indirectly to the secondary antibody - HRP. The Reagent 1 together with the Reagent 2 give rise to a reaction catalyzed by the enzyme HRP resulting in the emission of light. The signal can be detected through X-ray autoradiography. Very competitive price Highly sensitive, nonradiactive Superior signal and clear background BOCk/t A brand of Qu^fhem www.bqckit.com www.bioquochem.com