OxyCycler C42Dynamic O2 and CO2 Subchamber Controller • Highly reproducible O2/ CO2 exposures • Dynamic O2 / CO2 control • Limitless protocol possibilities • Physiological O2 / CO2 simulation • Pathologic O2 / CO2 modeling • Accountability • Automated control • PC interface DUAL CHAMBER / DUAL GAS The OxyCycler C42 is a unique dual channel/dual gas controller for incubators and C-Chambers. Oxygen and carbon dioxide control is managed via independently programmable channels for optimum control. No other instrument has such a fine simulation of dynamic exposure. Applications include intermittent hypoxia, graded hypoxia, and timed hyperoxia. OXYGEN CONCENTRATION Changes in O2 concentration do affect the physiology of the cells in vivo and now you can simulate these O2 dynamics in vitro. Complex, multi-setpoint oxygen profiles can be arranged to recreate conditions such as heart attack, strokes and epilepsy. When a profile is made, it can be reproduced to develop these dynamic models. The OxyCycler C42 is the predominant instrument capable of meeting these demands. CREATE ANY PROFILE The OxyCycler C42 controller is a unique instrument that offers sophisticated O2 and CO2 profiling. It easily creates any profile needed between 0.1-99.9% O2 and 0.120.0% CO2 independently and in multiple chambers simultaneously. The OxyCycler C42 enables multiple setpoints for any length of time, over any rate of change between these setpoints with any number of cycles and setpoints. The OxyCycler C42 makes complex oxygen cycling easy. MANY FEATURES A standard OxyCycler C42 comes with computer software for easy interface, real time trend charting, data logging, and remote operation. Since the OxyCycler C42 works with semi-sealed subchambers the profiles are normobaric. N2 and O2 gas infusions displace the chamber gas while internal and external pressure remain equilibrated.

Installation Schematic The OxyCycler C42 is used predominantly as a dual subchamber system for experiment and control. These isolating subchambers are placed within a third party CO2 (only) or thermal incubator. The OxyCycler C42 is supplied by three control gases: O2, N2, and CO2 and fits any of our standard subchambers. The OxyCycler C42 connects to the subchamber by flexible umbilicals, which consist of electrical and pneumatic leads, that are inserted into the incubator. Installation 1. Set OxyCycler C42 on or near host chamber and plug it in. 2. Hook up sensor and gas infusion tube and insert...

The OxyCycler C42 allows you to create any change in oxygen and carbon dioxide concentration. You can independently control and profile up to two chambers simultaneously, which means you have one control profile and one experimental profile. Up to seventeen different profiles can be stored and each can be run in any chamber at any time. Chambers can have the same profile simultaneously or can be staggered to begin at different times. Each profile can have 1 to 20 setpoints and can be from 0.1-99.9% O2 and 0.1-20.0% CO2. The rate between setpoints can be 0-999 minutes with resolution to seconds....

Sensor Operational Parameters HOST CHAMBER TEMPERATURE: 5-40°C HOST SENSOR HUMIDITY: 0-95%, Non-condensing 1. Controller: Bright digits display process values and provides manual user interface to control gas infusion. 2. Bleed Valves and Barbs: Bleeds gases out of gas supply lines. Calibration cup for sensor attaches here. 3. ZERO Calibration Gas Flowmeter: Used for calibration. 4. SPAN Calibration Gas Flowmeter: Used for calibration. 5. Needle Valves for Chamber 1: Sets infusion rate of control gases in each chamber to accommodate different dynamics. Can manually override controller to shut...