CO Subchamber Cell Culture System CytoCentric Products • First CO Gasotransmitter Cell Culture System • Precise Automated Exposures of CO • Dynamic or Static Exposures • Simulate Physiologic and Pathophysiologic conditions • Unprecedented CO Induced Phenotypes The GT series all use the same multipod adapter plate. This attaches to a C-Chamber and sits within your incubator. Single Chamber 0-400 PPM CO Control ADVANCED GASOTRANSMITTER INCUBATION Carbon monoxide (CO) is now known to be an exciting gasotransmitter involved in cell signaling, with powerful and interesting effects on cell phenotype. Until now, CO was difficult to study in cell culture because the only available method for applying CO in vitro used to be chemical donors. This method made it messy and difficult to control, and CO exposures were hard to reproduce. Now, with the OxyCycler GT4181C controller, cell culture in subchamber culture systems makes CO research easy, precise and very reproducible. UNLIMITED PROGRAMMED, DYNAMIC EXPOSURES Consisting of a GT41 which provides the common O2, CO2, and RH control, and the modular GT81C piggyback controller, which provides the CO, a subchamber culture system controlled by the OxyCycler GT4181C is the most advanced and reliable method for CO culture. Any dose and duration to cells in culture can be simply programmed. With the click of a mouse, any exposure can be precisely reproduced. Inside the culture chamber, all critical conditions are controlled by the OxyCycler GT4181C. Interface between the cells in culture and the GT4181C is completely protected by a microbial barrier. In addition, none of the sensors are directly exposed to the cells. This way, if the culture chamber gets contaminated, the sensors will not. Likewise, if the sensors ever get contaminated, they cannot contaminate your cells. MODULAR FLEXIBILITY The controller is compatible and will fit any standard size C-Chamber. The entire system will fit any existing incubator

Installation Schematic OxyCycler GT4181C Culture System. Controls dynamic or static O2, CO2, and CO, and limits RH in subchamber. Supply gases to GT41 required: Oxygen, Nitrogen, Carbon Dioxide. Calibration gas to GT41 required: certified pre-mix of 10% CO2 in balance oxygen. Supply gases to GT81C: non-certified pre-mix of 10000ppm CO in balance nitrogen. Calibration gas to GT81C required: certified pre-mix of 300ppm CO in balance nitrogen. Hot swap multipod adapter plate provides microbial barrier filters between cells culturing in the subchamber and all of the sensors and mechanisms that control...

Hypoxic stress can model components of many severe diseases such as heart attacks, strokes, asthma, or epilepsy. Frequency, duration and degree of drops are all adjustable. Cells destined for implantation will experience hypoxia and may be better prepared if they are conditioned to it before implantation. Ischemia may also be protected by conditioning. The OxyCycler GT4181C can easily run any preconditioning profile. Acute Hyperoxia Graded Hypoxia Model step reduction in oxygen, similar to altitude acclimation, to gradually condition cells for hypoxic upregulation of gene expression. The rate...

HOST CHAMBER TEMPERATURE: 5-40°C HOST CHAMBER HUMIDITY: 15-90%, Non-Condensing Sensor Operational Parameters Front and Back Panels 1. Bleed Valves and Barbs: Bleeds gases out of gas supply lines. Calibration cup for sensor attaches here. 2. Controller: Bright blue digits on black back ground. Continuously displays current control gas level, control status, and alarm status in all chambers. Displays menu items and settings during programming. 3. Alarm: Will sound if Ambient CO monitor detects unsafe levels 4. ZERO Calibration Gas Flowmeter: Used for calibration. 5. SPAN Calibration Gas Flowmeter:...