NO Subchamber Cell Culture System CytoCentric Products • First NO Gasotransmitter Cell Culture System • Precise Automated Exposures of NO • Dynamic or Static Exposures • Simulate Physiologic and Pathophysiologic conditions • Unprecedented NO Induced Phenotypes • Keeps Unwanted NO2 Side Product Within Limits The GT series all use the same multipod adapter plate. This attaches to a C-Chamber and sits within your incubator. Single Chamber 0-50ppm NO/NO2 Control ADVANCED GASOTRANSMITTER INCUBATION Nitric oxide (NO) is one of the most widely studied cell gasotransmitters, but traditionally has been difficult to work with in cell culture research. Until now the only way to dose NO in cell cultures previously was with a chemical that reacted to produce or “donate” NO. Chemical NO donors are messy and crude. Exposures possible with NO donors are limited. Every attempt is difficult to control and even more difficult to reproduce. Now, with the OxyCycler GT4181N, for the first time, you can precisely and automatically control NO. NO culture. Any dose and duration to cells in culture can simply be programmed, without the hassle and limitations of chemical donors. With the click of a mouse, any exposure can be precisely reproduced. UNLIMITED PROGRAMMED, DYNAMIC EXPOSURES Consisting of a GT41 which provides the common O2, CO2, and RH control, and the modular GT81N piggyback controller, which provides the NO, a subchamber culture system controlled by the OxyCycler GT4181N is the most advanced and reliable method for MODULAR FLEXIBILITY The controller is compatible and will fit any standard size C-Chamber. The entire system will fit any existing incubator. In addition, an unwanted side product that forms from NO and O2 is NO2. This nuisance gas can be prevented from building up in your culture chamber by automated sensing and displacement in real time to limit the concentration of NO2. This is essential for relevant and reproducible results of NO exp

Installation Schematic OxyCycler GT4181N Culture System. Controls dynamic or static O2, CO2, and NO, and limits RH and NO2 in subchamber. Supply gases to GT41 required: Oxygen, Nitrogen, Carbon Dioxide. Calibration gas to GT41 required: certified pre-mix of 10% CO2 in balance oxygen. Supply gases to GT81N: non-certified pre-mix of 1000ppm NO in balance nitrogen. Calibration gases to GT81N required: certified pre-mix of 30ppm NO in balance nitrogen and certified pre-mix 10ppm NO2 in balance nitrogen. Hot swap multipod adapter plate provides microbial barrier filters between cells culturing in...

Hypoxic stress can model components of many severe diseases such as heart attacks, strokes, asthma, or epilepsy. Frequency, duration and degree of drops are all adjustable. Cells destined for implantation will experience hypoxia and may be better prepared if they are conditioned to it before implantation. Ischemia may also be protected by conditioning. The OxyCycler GT4181N can easily run any preconditioning profile. Acute Hyperoxia Graded Hypoxia Model step reduction in oxygen, similar to altitude acclimation, to gradually condition cells for hypoxic upregulation of gene expression. The rate...

HOST CHAMBER TEMPERATURE: 5-40°C HOST CHAMBER HUMIDITY: 15-90%, Non-Condensing Sensor Operational Parameters Front and Back Panels 1. Bleed Valves and Barbs: Bleeds gases out of gas supply lines. Calibration cup for sensor attaches here. 2. Controller: Bright blue digits on black back ground. Continuously displays current control gas level, control status, and alarm status in all chambers. Displays menu items and settings during programming. 3. Alarm: Will sound if ambient monitor detects unsafe gas levels. 4. ZERO Calibration Gas Flowmeter: Used for calibration. 5. SPAN Calibration Gas Flowmeter:...