O2 and CO2 Controller for Live Cell Microscopy CytoCentric Products • Full Range O2 and CO2 • Real Time Control • Dual Stream - Control Gas and Liquid Phase • Eliminates Inflexibility of Premixed Gas • Eliminates High Cost of Premixed Gas Dual Stream 0.1-99.9% O2 and 0.1-20.0% CO2 • Eliminates Gas Stratification Inaccuracy • Vertical Footprint Saves Bench Space LIVE CELL MICROSCOPY OPTIMIZATION The OxyStreamer provides two identical streams of any mix of oxygen and carbon dioxide gas without the use of premixed gas cylinders. The two output streams can be used to simultaneously condition the atmosphere in microscope stage mount cell culture incubators/ chambers and the dissolved O2 and CO2 levels in media or perfusate. AVOID HASSLES & EXPENSE OF PRE-MIXED GAS By independently monitoring and controlling O2 and CO2 concentrations of the streams, the OxyStreamer can monitor and proportionally mix (blend) oxygen, carbon dioxide and nitrogen gases into any O2 and CO2 concentration in real time. Works with 3rd party live cell microscopy stage chambers, such as the DH-35iL Culture Dish Incubator shown above. FULL RANGE O2/CO2 OPTIMIZATION The OxyStreamer is easy to use. After calibrating the sensors, the desired concentrations for O2 (0.1-99.9%) and CO2 (0.1-20.0%) are simply entered into their respective controllers. The closed-loop dynamic gas control system will then automatically monitor and adjust the output stream of gas to the pre-programmed gas ratios in the controllers. This technique will maintain the output mixed gas concentration to within 0.2% of the selected setpoint levels. LIQUID & GAS PHASE SIMULTANEOUSLY The flow rate for each stream is independently controlled for gas efficiency to provide a reproducible rate at which the gas is delivered to the cell culture chamber and/or diffused into the cell culture media or perf

Options include a Windows® based software package that provides trend plotting, data logging, and remote operation via RS connection to your PC. Multiple OxyStreamers can be daisy-chained via optional RS-485 interface. 1. Controller: Bright digits display process values and provides manual user interface to control gas infusion. 2. Alarm Switch: Toggles the alarm function on and off. 3. Calibration Bleed Barbs: Used to calibrate sensors. 4. Bleed Valves and Barbs: Bleeds gas. 5. Stream Flowmeters: Adjusts flow rate of streams. 6. Alarm Receptacle: Connect an appropriate alarm of your choosing...