ProOx C21
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Compact O2 and CO2 Subchamber Controller CytoCentric Products • Full range O2 control • Independent CO2 control • Contamination resistant • Forced gas homogenization • Combines the best features of O2 and CO2 controllers in one unit Single Chamber 0.1-99.9% O2 and 0.1-20.0% CO2 The ProOx C21 is a practical tool for researchers experimenting with cell cultures where both O2 and CO2 control and monitoring is needed. It controls both gases in one convenient unit. FITS MOST CELL CULTURE CHAMBERS The ProOx C21 fits and controls most bench-top incubators and a variety of common cell culture chambers. The ProOx C21 is compatible with the BioSpherix C-Chamber (subchamber). The unit is installed quickly and easily. PHYSIOLOGIC AND PATHOLOGIC O2 Cells normally exist in low oxygen micro-environments and thus need to be modeled in vitro. The ProOx C21 is able to simulate any low oxygen environment. The unit can also supply elevated oxygen concentration for hyperoxic conditions. It independently controls CO2 for correct pH maintenance. The gases are infused and controlled to the user programmed set point. ProOx C21 consists of two basic parts connected by an umbilicus: the controller itself and its adapter pod that attaches to a C-Chamber. CONTROL IS EFFICIENT Feedback from the custom designed O2 and CO2 sensors tells the ProOx C21 the time and amount of gas to infuse. Thus, no gas is ever wasted. Recovery after access is especially rapid. DECREASES RISK OF CONTAMINATION The remote gas pod has a unique design that is resistant to initial and inadvertent cross-contamination. The pod works by isolating the potential source of the contamination, using disposable microbial barriers on all parts of the gas delivery system. If the sampling loop becomes contaminated the microbial barrier eliminates the risk of cross infection on the return. All of the controlled, infused gas is sterile filtered. The microbial barrier filters are user-replaceable. All contact surfaces, including the gas homogenization fan, are easily decontaminated. HANDLES ANY JOB • Where ambient conditions are not correct, the ProOx C21 can provide any other level of O2 or CO2 you need. • Where ambient atmosphere is not air, the ProOx C21 can restore and maintain air-equivalent O2. • Where O2 or CO2 consumptive or generative processes need to be countered the ProOx C21 can hold both stable against destabilizing loads. It can work continuously year-round, or on occasion as

Installation Schematic N2 From outside the host chamber, the ProOx C21 works by remotely sensing oxygen and carbon dioxide inside the chamber and infusing gas to control both. Oxygen and carbon dioxide are infused to raise each of their levels and nitrogen is infused to lower them through displacement. Once the initial gas reaches its set point, the second quickly follows. The accessory pod that attaches to the sub chamber contains the gas sensors. These sensors are isolated behind microbial barriers and also isolated from the controlled environment of the culture chamber. The pod also...

At time (1) user opened and closed door briefly for access. Room air rushed in and took both O2 and CO2 away from setpoint. However, automatic feedback immediately responded and returned both gases back to setpoint. At time (2) user unlatched door but before he could open it became distracted and forgot to re-latch, thus increased chamber leakage. Again however, automatic feedback control quickly adjusted and re-established setpoints. Obviously gas consumption increased to accommodate the increased load but the integrity of the experiment was maintained. SETPOINT OXYGEN TIME CONTROL GAS IS...

HOST CHAMBER TEMPERATURE: 5-40°C (depending on sensor) HOST CHAMBER CO2: 0.1-20% (depending on sensor) HOST CHAMBER HUMIDITY: 0-95% RH. Non-Condensing 1. Bleed Barbs: Used in calibration. Calibration cup for sensor attaches here. 2. Bleed Valves: bleeds gases out of gas supply lines. 3. Controller: bright blue digits on black back ground. Continuously displays current control gas level, control status, and alarm status in all chambers. Displays menu items and settings during programming. 4. Gas Switch: Manually overrides controller to shut off gas. 5. Alarm Switch: Manually overrides...