Crosslinker Spectrolinker XL - 1000 / XLE 1000
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Crosslinker Spectrolinker XL-1000 / XLE 1000 Spectrolinker — the World's Most Advanced, Versatile and Accurate UV Crosslinker is Also the Easiest to Use! The Spectrolinker was designed to be one of the most useful, multi-purpose instruments you can use in the lab. We built it with a "smart" microprocessor controller and unique true-UV-monitoring circuitry to enable you to get error-free results with greater speed, accuracy and safety than was previously possible. That's why we also eliminated the needlessly time-consuming adjustments, unnecessarily repetitive programming and unwarranted risk of sample damage you encounter with conventional crosslinkers and other UV equipment such as light boxes and transilluminators. Our ultimate goal is to make this revolutionary new instrument your most indispensable apparatus. 154201-001 154203-001 154211-001 154213-001 Auto Repeat “Remembers” Previous Settings for Quick and Simple, One-Button Operation You never have to enter the same numbers over and over. Just press “Start” to automatically repeat or to resume a userinterrupted operation. Should the door be opened during operation, the Spectrolinker alternately flashes “door” and the remaining time or energy. The “Reset” button cancels the latest settings. Unparalleled Accuracy Our state-of-the-art photo sensor provides true UV monitoring. Competitors’ units employ inferior, bare photodiodes, which render them inaccurate by reading unwanted visible and infrared radiations. The Spectrolinker’s exclusive, wavelength-specific, multi-stack UV photo sensor, which is factory-calibrated to NIST (NBS) specifications, provides the most precise UV-only dosage measurements. 254nm tubes are standard, with 312nm or 365nm tubes optional. Full-rangedisplay resolution is accurate to ±5µW/cm2. EMI/RFI protection. Super-Fast Crosslinking Binds Samples in Under 30 Seconds. Vacuum-oven baking would take over two hours at 80°C. The Spectrolinker is over 240 times faster! Safeguards Your Valuable Test Results and Optimizes Binding After investing your valuable time and effort, the last thing you want is to have your improperly bound sample disappear during the wash cycle. No need to worry about that with the Spectrolinker! Just select the desired energy level and its unique true-UV-monitoring circuitry assures that the dosage you set is always precisely delivered. Even when the tubes age or if the AC-line power fluctuates, the crosslinker’s automatic photoelectric feedback circuitry assures that your samples will be properly irradiated. And its “smart” microprocessor controller, large LED display and color-coded key pad reduce human error. An anodized-aluminum chamber prevents sample contamination due to rust. Dramatically Enhances Hybridization-Signal Sensitivity as compared to conventional methods Tests were conducted at Cold Spring Harbor Laboratory (NY) to determine what effect, if any, different binding methods have on DNA retention. The autoradiographic results (on right) conclusively prove that UV irradiations with the Spectrolinker significantly improves retention over vacuum-oven baking. Three samples each of single-stranded, M13 DNA were applied in the 1) 2) amounts shown in the autoradiograms in GeneScreen nylon and 0.45µm nitrocellulose membranes by slot blotting. One membrane from each set of three was processed by vacuum baking for two hours at 80°C. The second and third membranes were UV irradiated by the 2 2 Spectrolinker at 120mJ/cm (optimal) and 90mJ/cm dosages, respectively, and then hybridized 3) to an RNA probe (in five-fold excess) according to Maniatis et al . DNA retention was quantified as counts per minute (CPM) of the hybridized probe by an Ambis Radio-analytic Imaging 4) System 1. 2. 3. 4. Manufactured by and Trademark of DuPont New England Nuclear, Boston, MA Manufcatured by Schleicher & Schuell, Keene, NH J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 2nd Edition, 1989. Manufactured by Am