Processing Lab: Via Giovanni Agnelli, 3 10020 Riva Presso Chieri (TO) - Italy Type I bone collagen interacts with the beta 1 subunit of osteoblast cell surface integrins, thus promoting cell adhesion to graft material; Bioteck is the leading Italian company in the production of heterologous grafts for neurosurgery, orthopaedics, oral and maxillo-facial surgery. It was founded by the end of the 90’s and it is constantly growing thanks to its exclusive system of enzymatic deantigenation at 37°C. Completely safe grafts with advanced biological and biomechanical characteristics are obtained from this system. acts as a coactivator needed for the action of morphogenetic proteins (BMPs), thus fostering the stimulating action of endogenous growth factors; binds soluble growth factors by turning them into insoluble factors: therefore, they are protected from proteolysis and their half-life is increased, extending the duration of regenerative stimulation; regulates the access of extracellular factors to new-formed bone crystal, thus physiologically modulating bone mineralization; modulates the transduction of the proliferation and differentiation signal in osteoblastic cells, thus regulating the remodeling process; interacts with the mesenchymal cells stemming from bone marrow, by inducing the adhesion, the proliferation and the differentiation in osteoblasts; promotes bone regeneration whenever grafted in case of bone defects, having a direct action of regeneration promotion; stimulates the expression of gene coding for BMP type II receptor, thus making cells more sensitive to regenerative signals. type I collagen effects concept: mauro forlani - vi Bioteck S.p.A. Headquarter: Via E. Fermi, 49 - 36057 Arcugnano (VI) - Italy Phone +39 0444 289366 - fax +39 0444 285272 [email protected] - www.bioteck.com print: abalti.it typeIcollagen safetybiocompatibility Bioteck currently produces and distributes in more than 50 countries: Biocollagen - membranes and pastes based on type I and III collagen from equine Achilles tendon. Osteoplant - a wide range of both cancellous and cortical bone grafts. Osteoplant Flex - a line of exclusive grafts partially demineralized, making them mouldable and flexible. Osteoplant Activagen e Angiostad - injectable and mouldable bone pastes in syringes, with huge osteoconductive and osteopromotion properties. References 1 - S. Pagnutti, S. Maggi, D. A. Di Stefano, M. Ludovichetti “An Enzymatic Deantigenation Process Allows Achieving Physiological Remodelling and Even Osteopromoting Bone Grafting Materials” Biotechnol. & Biotechnol. Eq. 21/2007/4 2 - G. Bellone, T. Scirelli, G. Emanuelli “Osteo-promoting activity of Osteoplant Angiostad in vitro.” Minerva Stomatol. 2008;57:189-98 3 - Mizuno M, Fujisawa R, Kuboki Y. “Type I collagen-induced osteoblastic differentiation of bone-marrow cells mediated by collagen-alpha2-beta1 integrin interaction.” J Cell Physiol. 2000 Aug;184(2):207-13 4 - Gungormus M, Kaya O. “Evaluation of the effect of heterologous type I collagen on healing of bone defects.” J Oral Maxillofac Surg. 2002 May;60(5):541-5 5 - Toroian D, Lim JE, Price PA. “The size exclusion characteristics of type I collagen: implications for the role of noncollagenous bone constituents in mineralization.” J Biol Chem. 2007 Aug 3;282(31):22437-47 6 - Bellone G, Scirelli T, Emanuelli G. “Osteoinductive activity of Activagen in vitro.” (Submitted for publication) Biocollagen crunch covering the proximal part of a revision femoral stem which is excessively exposed because of the huge loss of bone substance. Cortesy of Dr. Renzo Bianchi, Ospedale Civile, Feltre (Belluno) - Italia. Activagen mouldable paste combined with cancellous chips and autologous bone marrow concentrate. The graft is mixed and a cut-off syringe is filled with it. It is inserted into the bone gap under the metal plate. Cortesy of Dr. Ferdinando Da Rin, Istituti Codivilla-Putti, Cortina d’Ampezzo (Belluno) - Italia. 7 - Choi Jong-li, et al. Korea Atomic Research Institute. Jeongup 580-185 Republic of Korea “Effect of ionizing irradiation on osteoinductivity of demineralized bone matrix” Poster EATB 2009 Krakow PL biocollagen gel & crunch osteoplant

bonepaste Bioteck demineralization of extra-cellular matrix Bioteck bone pastes are obtained from equine bone tissue through an exclusive physicochemical process of enzymatic deantigenation and subsequently of total demineralization of bone matrix. Using digestive enzymes, which work at physiological temperature (37°C), makes it possible to completely remove the tissue antigenic component without changing the native conformation of collagenous matrix molecules, which is therefore totally preserved. The deantigenated tissue is subjected to a total demineralization process. Calcium salts are completely...