Biochemical Laboratory/Quality Control - Bioteck S.p.A. Bioteck S.p.A. Headquarters: Via E. Fermi 49 - 36057 Arcugnano (VI) - Italy Tel. +39 0444 289366 - fax: +39 0444 285272 [email protected] - www.bioteck.com The DNA content is directly proportional to the cell population present in the tissue. The “control” is untreated pericardium. The other columns show the constant reduction of residual DNA following the first treatment cycle according to the Zymo-Teck® process. Further cycles help guarantee complete deantigenation. % LIPID CONTENT Quantification of the lipid content Production and R&D Center: Via G. Agnelli, 3 - 10020 Riva presso Chieri (TO) - Italy Bioteck® is an Italian company producing bone substitutes and protective membranes that are successfully used in orthopaedics, neurosurgery, oral and maxillofacial surgery. Founded in 1995, the company continues to grow constantly and now operates in more than 50 countries around the world. A firm commitment to scientific research forms the basis for the innovative solutions offered by Bioteck® products. The company collaborates on numerous national and international research projects, which have driven the basic research and helped in writing important chapters in bone biology. The in-depth knowledge acquired by Bioteck® through its research ensures the absolute quality of its products, which are subjected to strict environmental and quality controls, thereby guaranteeing a product meeting the highest quality and safety standards. Bioteck® applies a policy of total transparency, opening up the doors of its Production and R&D Center for the monitoring of its innovative manufacturing process and the intense Production and R&D Center scientific research carried out by its staff. concept: mauro forlani - vi - cod. 141024_N_HR_C_EN01-00 - printed by: abalti.it Quality and safety guarantee The “control” is untreated pericardium. The other columns show the drastic reduction of the lipid content after the first treatment cycle according to the Zymo-Teck® process. Biochemical Laboratory/Quality Control Heart® - an equine pericardium membrane range for neurosurgery, orthopaedics, general surgery, oral and maxillofacial surgery. Heart® and Zymo-Teck® are registered Bioteck S.p.A. trademarks Electrophoretic separation on denaturing gel (SDS-PAGE). The first column (MWM) shows the molecular weight markers, the second (+), a type I collagen sample treated with pepsin, an enzyme able to cut the proteins at specific points. The third column shows a sample Heart® membrane subjected to the same treatment. The bands visible in the column (+) are entirely comparable with those seen in the Heart® membrane, confirming the presence of collagen in its native conformation.

Application of Heart DM in repairing a dural defect ® biological matrix for dural replacement Zymo-Teck Process: the secret of the quality of grafts ® The Heart® DM membrane observed under the scanning electronic microscope (SEM) has a multilayer, compact appearance, characterised by a close-knit weave of collagen fibres. Padua University, Biology Department, Electronic Microscopy Service. Heart® DM is a biological matrix for dural replacement and repair, obtained from carefully-selected equine pericardium, to ensure just the right quality and thickness, and which is thereafter treated using...