Standardised procedures for the microbiological analysis of foodstuffs in accordance with ISO standards

Food Microbiology Scharlab has accumulated over 35 years of experience in the production of microbiology culture media. In 1991, we also started to sell ready-to-use culture media to satisfy the market’s changing preference for such products instead of dehydrated culture media. Scharlau’s portfolio of culture media includes media specifically created for quality control in different sectors: · Pharmaceutical and veterinary industries · Cosmetics · Food and beverage sector · Water analysis and environmental laboratories · Hospitals and clinics All of our dehydrated and ready-to-use culture media...

Scharlau The wise choice The COMMISSION REGULATION (EC) No. 1441/2007 amending REGULATION (EC) No. 2073/2005 of 5 December 2007 on microbiological criteria for foodstuffs establishes: I. The microbiological criteria for certain microorganisms and the rules food business operators must follow when implementing the general and specific hygiene measures set out in Article 4 of Regulation (EC) No 852/2004. II. That food business operators must guarantee that foodstuffs comply with the relevant microbiological criteria set out in Annex I of Regulation (EC) No. 2073/2005. Furthermore, Regulation (EC)...

Food Microbiology The wise choice Total enumeration • ISO 4833-1 Microbiology of the food chain.Horizontal method for the enumeration of microorganisms. Part 1: Colony count at 30°C by the pour plate method. This part of ISO 4833 describes a horizontal method for the enumeration of microorganisms that are able to grow and form colonies in a solid media after aerobic incubation at 30°C. W Mechanically separated meat. linnj Sample Streaking and inoculation: W Transfer 1 ml of the test sample, if liquid, or 1 ml of the initial suspension (10-1 dilution) to two sterile, empty Petri dishes. W Transfer...

Food Microbiology Scharlau The wise choice This international method specifies a horizontal method for the detection of Salmonella, including Salmonella typhi and Salmonella paratyphi, and can be applied to: Microbiology of food and animal feeding stuffs. W Minced meat and meat preparations intended to be eaten raw. W Minced meat and meat preparations made from poultry meat intended to be eaten cooked. W Egg products and foodstuffs containing raw egg. W Cooked crustaceans and molluscan shellfish. W Live bivalve molluscs, echinoderms, tunicates and marine gastropods. W Ready-to-eat pre-cut fruits...

Food Microbiology The wise choice Coagulase-Positive Staphylococci • ISO 6888-1/2 Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1: Technique using Baird-Parker agar medium. Part 2: Technique using rabbit plasma fibrinogen agar medium (RPF). ISO 6888 describes two horizontal methods (Part 1 and Part 2) for the enumeration of coagulase-positive staphylococcus among which enterotoxigenic strains are encountered. Mainly concerned with Staphylococcus aureus, but also ^ Staphylococcus...

ISO 7932 describes a horizontal method for the enumeration of the viable presumptive Bacillus cereus by means of the colony-count technique at 30°C. W Dried infant formula and dried dietary foods for special medical purposes intended for infants below six months of age. Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of presumptive Bacillus cereus. Colony-count technique at 30°C. Make an initial suspension and its dilutions (1:10) for streaking. Streaking and inoculation: W Transfer 0.1 ml of the sample, if liquid, or 0.1 ml of the initial suspension to two...

The wise choice Scharlau’s plastic consumable material for the application of ISO standards Threaded containers for samples PP urine containers with PE cap. Writing surface on the side. Wide thermal and chemical resistance. The sterile models have been irradiated and are supplied in an individual bag. Two capacities available. The code PUC1501000, is delivered with the cap without threading. Cylindrical flasks (Duchess type) Duchess type flasks, cylindrical with wide neck, translucent white, made of high density polyethylene. For the collection, conservation and storage of samples. With black...

Food Microbiology Microbiology of the food chain. Method applicable to: w Chicken broiler carcasses. Part 2: Colony-count technique. Horizontal method for detection and enumeration of Campylobacter spp. CCDA Campylobacter jejuni ATCC 29428 Dilutions and streaking: w From the sample, prepare serial dilutions in BPW. w Streak 0.1 ml of the initial suspension, and the serial dilutions if necessary, in Modified CCD Agar (Charcoal Cefoperazone Deoxycholate). Enumeration: w The typical colonies of Campylobacter spp. are greyish and often have a metallic sheen, and are flat and moist, with a tendency...

Food Microbiology The wise choice Listeria monocytogenes • ISO 11290-1 Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes. Part 1: Detection method. Part 2: Enumeration method. ISO 11290-1 describes a horizontal method for the detection of Listeria monocytogenes, a pathogen that can be found in food. W Ready-to-eat foods intended for infants and ready-to-eat foods for special medical purposes. W Ready-to-eat foods able to support the growth of Listeria monocytogenes, other than those above. PROCEDURE Primary enrichment (preparation of...

Food Microbiology Scharlau ISO 11290-2 describes a horizontal method for the enumeration of Listeria monocytogenes, a pathogen that can be found in food. Method applicable to: W Ready-to-eat foods able to support the growth of Listeria monocytogenes, other than those intended for infants and for special medical purposes. Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes. Part 1: Detection method. Part 2: Enumeration method. PROCEDURE Preparation of the initial suspension (primary enrichment): W Add 25 g or ml of the sample to 225 g or...