Legionella Control Legionella In recent years, the growing awareness of the health risks associated with this bacterium has driven significant regulatory changes. The publication of ISO 11731:2017 has standardised at an international level the methods for detecting and enumerating Legionella spp., improving the reliability and comparability of analytical results. Proper microbiological analysis, carried out in accordance with current regulations, is an essential pillar in ensuring facility safety, protecting public health, and complying with legal requirements. These are Gram-negative bacilli....

Legionella Control Legionellosis is a notifiable disease and of public health significance. Its prevention and control are governed by detailed and specific local and international regulations. Water quality - Enumeration of Legionella by culture method. Official standard for the isolation and quantification of Legionella in water and associated materials. Method adopted under RD 487/2022 and revised by RD 614/2024. Test method for evaluating the bactericidal activity of disinfectants against Legionella. Applies to products used in water systems: cooling towers, showers, hot tubs, etc. Establishes...

Legionella Control Gcharlab THE LAB SOURCING GROUP Sampling shall be carried out in accordance with documented procedures included in the sampling programme. The sampling programme must include, at a minimum: the microbiological and physico-chemical parameters to be monitored; identification of the sampling points; frequency or timing of sampling; the number and type of determinations to be performed; sampling methods; conditions for sample preservation and transport; testing methods; criteria for evaluating results; and the designation of those responsible for each operation. Description Pack....

Legionella Control To ensure effective detection of Legionella spp. in water samples, ISO 11731 specifies that, in most cases, a concentration technique using membrane filtration should be applied. This technique is especially essential when the Legionella concentration in the sample is unknown or suspected to be low. Filtration with direct placementof the membrane on the culture mediumMembrane type • Material: cellulose nitrate or mixed cellulose esters • Use: direct placement onto the medium after filtration • Must comply with ISO 7704. • Use of black filters is recommended, as they improve...

Scharlab Legionella Control Sample pre-treatment (CH. 8.3) Procedure carried out to reduce the accompanying microbiota without affecting Legionella. Heat treatment Acid treatment Procedure: Procedure: Legionella is highly nutritionally demanding and does not grow on standard culture media. It requires ferric salts and L-cysteine. The ISO standard establishes a range of media depending on the concentration of Legionella and the presence of interfering flora in the analysed sample. 1. Take 1 ± 0,5 ml of the sample or concentrate. 2. Heat in a water bath at 50 ± 1°C for 30 ± 2 minutes. 3. Strict...

Legionella Control Culture media (CH. 6) (continuation) Culture media (continuation) Other related media: MWY Agar (Modified Wadowsky Yee Agar). Highly selective medium with Glycine, Polymyxin B, Vancomycin, Anisomycin, and two indicators (Bromothymol Blue and Bromocresol Purple). Columbia Blood Agar Description BCYE Agar Base BCYE Agar Base MWY Selective Supplement BCYE-Cis Agar Pack. 500 g 5x500 ml 10 vials 20 plates of 90 mm Other related media: TSA (Tryptic Soy Agar) Reference 01-200-500 01-200BA05 064-PA0031 064-PF0026 064-BA1008 064-BA6043 Pack. 500 g 5x500 ml 20 plates of 90 mm 30 plates...

Legionella Control Confirmation of presumptive Legionella colonies (CH. 8.5) Select presumptive colonies from the plate with the highest count. • If there is only one morphological type, pick 3 colonies. • If there are several morphological types, pick at least 1 colony of each type. Subculture each colony onto two plates: one with BCYE (with cysteine) and one with BCYE-cys (without cysteine). Inoculate first on BCYE-cys, then on BCYE (alternative media such as blood agar and nutrient agar may be used). Incubate at 36 ± 2°C for 2–5 days. Colonies that grow on BCYE but not on BCYE-cys are confirmed...

Legionella Control Culture medium Control strains References MiStraCon® Nutritivo Agar, Confirmation Sangre Agar, TSA White-grey-blue-purple colonies with BCYE Quantitative PR > 0,5 entire edges and a characteristic frosted glass appearance Legionella anisa ATCC® 35292™ S00026-MSC <2> Qualitative or partial inhibition Legionella pneumophila ATCC® 33152™ S00017-MSC (2) BCYE agar White-grey-blue-purple colonies with entire edges and a characteristic frosted glass appearance 111 Strain to be used at a minimum 121 Strain only available in qualitative format (3) At least one of the two must be used...

Legionella Control Sample type Procedure 5 Media A and Procedure 7 Media B or C Direct placement of the membrane onto culture medium (8.4.3.1) Untreated: BCYE Acid treatment: BCYE+AB or GVPC or MWY Record filtered sample volume Sample collection bottle Cellulose membrane Acid solution BCYE BCYE+AB / GVPC / MWY Confirmation with BCYE + cysteine-free BCYE Latex test ^ *Media on pages 6 and 7 ^ Bottles on page 4 ^ Membranes on page 5 ^ Latex test on page 8 Samples with LOW Legionella concentration / LOW interfering microorganisms concentration Procedures 8, 9 and 10 in Media A and in Media B or...

Legionella Control Sample type Decision matrix Culture media ^ *Media on pages 6 and 7 ^ Bottles on page 4 ^ Membranes on page 5 ^ Latex test on page 8 Filter type [email protected] • www.scharlab.com Scharlab the lab sourcing group 11

Legionella Control ISO 11731: 2017. Enumeration of Legionella Scharlab can offer you the full range of equipment needed for the Legionella counting process. Please don’t hesitate to contact us! Biological safety cabinets Colony counters Filtration manifolds Ultrasonic baths Ultraviolet lamps Vacuum pumps Vortex mixers Water baths Scharlab Philippines, Inc. 4/F Unit K, No. 35 Sto. Niño Street cor. Fernando Poe Jr. Ave. Barangay San Antonio, Quezon City 1105 Philippines Landline: +63 2 8671 9674 E-mail: [email protected] Scharlab Italia S.r.l. Via Massimo D’Azeglio 20 26900 LODI (LO)...