Advanced Substrate Holder (ASH-100) for Serial Section Array Tomography This multi-axis manipulation tool developed by researchers at the Karlsruhe Institute of Technology and the Cryo EM Centre, Bioquant, University Clinic Heidelberg enables ribbons of ultrathin sections to be collected directly onto a conductive substrate from the ultramicrotome diamond knife. Shown and demonstrated here with the RMC-Boeckeler ATUMtome the substrate holder is mounted in front of the ATUMtome Ultramicrotome on its x-y positioning slide rail. For this demanding application a high performance vibration isolation table is recommended, as supplied with the ATUMtome. We also recommended the custom designed, large boat, Diatome diamond knife seen in this illustration. For Array Tomography at nano-scale resolution this non-destructive method preserves the sectioned samples enabling correlative imaging. Different imaging techniques such as light microscopy (LM) and electron microscopy (EM) are applied on the same sample. Features: 1. Seven adjustable axes for precision positioning of the substrate in the diamond knife water trough 2. Lift up micro positioning enables the operator to collect multiple ribbons of sections on the same substrate 3. Compatible with ATUMtome automated tape collecting Ultramicrotome and may be set-up side by side on the same vibration isolation table system and mounting rail 4. Available substrates include: 5. Indium tin oxide (ITO) coated glass coverslips 6. Conductive silicon wafer segment

1. Lock - Z 2. Raise-Lower (Z) 3. Lock - X 4. X - Fine Adj 5. Micrometer - Fine Rotation 6. Clutch - Fine Rotation 7. Fine Rotation Stop 8. Lock - Rail (X) 1. Substrate Advance Retreat 2. Lock - Substrate Advance Retreat 1 Images courtesy of Waldemar Spomer 4650 S. Butterfield Drive • Tucson, Arizona 85714 USA Phone: 520.745.0001 • Fax: 520.745.0004 • E-mail: [email protected] • www.rmcboeckeler.com © 2015 Boeckeler Instruments, Inc. Boeckeler is a registered trademark of Boeckeler Instruments, Inc., Tucson, Arizona. All other trademarks are the property of their respective owners. All specifications...