□ The Original and Proven High Pressure Freezing Machine □ Over 100 Installations World-Wide □ More Than 400 Scientific Publications □ Well Frozen Specimens up to 200pm Thickness and 2mm Diameter, Without the Use of Cryoprotectants High pressure freezing is the only method, that allows vitrification of samples up to 1mm3. The HPF method uses LN2 under high pressure (2100 bar). At 2100 bar, water is 1500 times more viscous than at atmospheric pressure, which drastically reduces the crystal growth. HPM 010 High Pressure Freezing Machine Features • Freezing of specimens up to 200pm thickness and 2mm dia. without visible ice crystal damage using standard type A or B 6mm diameter specimen carriers • Cryo-fixation of suspensions, monolayer cell cultures and tissues • Large base of application know-how available • Shortest handling time before freezing • Suited for suspension and tissue samples • Reproducible freezing • Easy one button operation • In situ real time measurement of temperature and pressure. • Fast 90 second process cycle allows expeditious application. • Automatic, microprocessor controlled operation for routine work. • Compact, sturdy unit with soundproof and vibration-free housing. • Simple and safe operation due to quick-locking action of specimen holder and clearly arranged operational controls. • Processing data is recorded on digital display, such as the actual temperature, time and pressure, thus allowing the user to exactly evaluate current operational status (sample quality control). • Simple maintenance with removable cover plates and rack system for control units. • Extensive accessory program. Cross section of cells after chemical fixation Trichocyst bodies (TB) is detatched all round from the matrix. Glykogengranula is mixed with the Ribosomes. High pressure frozen, freeze substituted Preparation in Cellulose Capillary tubes No discernible shrinkage of the Trichocysts. Bodies and tips are firmly connected. Glykogengranula (GLY) is delimited from the Ribosome-areas (RI). BMC Boeckeler A division of Boeckeler Instruments, Inc. 4650 S. Butterfield Drive , Tucson, Arizona 85714 USA

Weight, approx. Workin g Data Working pressure Maximum pressure Duration of working pressure, at least Cooling time from 0°C to -50°C (measured between 3 mm copper disc) Sp e cim en Di mens i ons Sample size, up to max. of Con nect i on Dat a Voltages, frequencies Power input, approx. Heating water (rubber hose) Initial system cooling, approx. Max. processing sequence, approx. lsopropyl alcohol reservoir, approx. Specimen holder Specimen pressure chamber Quick fastening bolt Specimen LN2 entry apertures Pressure sensor Temperature sensor N2 exhaust with silencer Outlet apertures lsopropyl alcohol...