HPM 010
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HPM 010 Boeckeler High Pressure Freezer The Original, Proven, High Pressure Freezing Machine for Larger Samples The HPM 010 provides sustained high pressure during the freezing cycle due to its hydraulic system (it is not pneumatic!). Sustained high pressure freezing yields good freezing quality throughout a 100 - 200 micrometer thick specimen (published). Excellent freezing of the specimens intended for examination in the electron microscope is one of the most important prerequisites for achieving reproducible results from the various subsequent cryo preparation methods. The freezing method should produce microcrystalline or amorphous ice from the specimen water. To achieve this, the specimens must be frozen as quickly as possible at a freezing rate no lower than 10,000 C/s. The conventional freezing methods in use are plunge freezing, jet spray and cold block (slamming) cryo fixation. However, due to the poor heat conductance of water, these methods can only satisfactorily freeze specimens measuring up to between 10 and 20pm. Thicker specimens (such as tissue samples) could only be frozen in the past, if a cryoprotectant was added to lower the freezing point of the water in the specimen. The disadvantage of chemical cryoprotectants is that they often affect certain cell structures, causing different types of undesirable artifacts. FEATURES ♦ Over 100 installations world-wide with well-established user group ♦ More than 400 scientific publications ♦ Well frozen specimens up to 200pm thickness and 6mm diameter, without the use of cryoprotectants using standard type A or B 3mm and 6 mm diameter specimen carriers ♦ The HPM 010 provides excellent results with tissue samples and, of course, suspensions. ♦ Array of specimen carriers available, i.e. for suspensions (capillary tubes), tissue extracted by fine needle biopsy and carriers for monolayer cell cultures. 4650 S. Butterfield Drive • Tucson, Arizona 85714 USA E-mail: info@boeckeler.com • www.rmcboeckeler.com © 2015 Boeckeler Instruments, Inc. Boeckeler is a registered trademark of Boeckeler Instruments, Inc., Tucson, Arizona. All other trademarks are the property of their respective owners. All specifications subject to change without notice.

Dimensions and Weight Dimensions Weight, approx. Working Data Working pressure Maximum pressure Duration of working pressure, at least Cooling time from 0C to -50C (measured between 3 mm copper disc) Specimen Dimensions Sample size, up to max. of Connection Data Voltages, frequencies Power input, approx. Compressed air (5 bar) LN2 (1 bar excess pressure) Heating water (rubber hose) Operational Data Hydraulic oil reservoir Hydraulic oil bias pressure LN2 Dewar (in system) LN2 consumption Initial system cooling, approx. Max. processing sequence, approx. lsopropyl alcohol reservoir, approx....