• Accelerate Biopharmaceutical Analysis Innovation with Integri

Developed with the Biopharma Industry Award-winning biopharmaceutical analysis platform Commercial and regulatory pressures are having significant impact on the analytical workload of many discovery and development groups. The ability to provide accurate knowledge quickly and confidently without fear of omissions is key. Due to its versatility, Mass Spectrometry (MS) has evolved into the “go-to” technique for addressing a multitude of characterization and process development challenges. BioPharma Compass* software provides biopharmaceutical scientists working in drug discovery and development...

Intact Protein Profiling and Verification Intact Protein Analysis: Glycoforms and Heterogeneity Screening The ultra-high resolution available on Bruker’s maXis II UHR QTOF has been proven to be uniquely beneficial to the identification of low level protein isoforms in mAb characterization1. With Bruker's patented SNAP algorithm and True Isotopic Pattern (TIPTM) calculation, the automatic assignment of molecular formulae of monoisotopic masses up to 50kDa with sub-ppm precision is possible. The cosine similarity score, which provides an automated quality assessment, simplifies batch comparison....

Simple. Yet Versatile and Powerful Glycans Correct primary structure assessment Correct primary structure assessment and extensive glyco-profiling of cetuximab and extensive glyco-profiling of cetuximab by a combination of intact, middle-up, bymiddle-down andof intact, middle-up, a combination bottom-up ESI and MALDI middle-down and bottom-up ESI and MALDI mass spectrometry techniques mass spectrometry Jabs, * Anja Resemann, techniques Daniel Ayoub, * Wolfgang 1, Waltraud Evers, 2 Catherine Evans, 3 Laura Main, 4 Daniel Ayoub,1, Wolfgang Jabs, 2 Anja Resemann, 2 Waltraud Evers, Catherine Evans,...

Peptide Mapping for Sequence Confirmation and Attribute Identification BioPharma Compass simplifies viewing and reporting peptide mapping results. Clear overview and detailed validation screens are available for high quality sequence confirmation and post-translational modifications (PTM) identification. Figure 3: Tryptic digest analysis of the NIST mAb: LC (UV or BPC) dataset confirming expected peptides, highlighting unexpected peptides Peptide Screening - Attribute Monitoring and Quantification With BioPharma Compass it is easy to seamlessly generate screening lists from peptide mapping results...

Workflow Selection Automated A and Process as Protein Sequence Editor Import MS-Data Protein Screenin @ Queue Bate , Peptide Mapping / PTM Comparison Peptide Screening / Batch Comparison 1 Bruker Blue Ribbon Menu allows control of workflow specific appearance and easy initiation of analyses 2 Project View with all associated files 3 Main View with identified peptides / proteins 4 Mass Spec View / Chromatogram View 5 Protein sequence information

Data Review

Accelerate Biopharmaceutical Analysis Summary • Integrated biopharmaceutical workflows from acquisition to reporting for automated characterization, identity confirmation, attribute monitoring, comparative quantitative assessments -- Protein Screening: Intact, reduced, subunits, including glycan profiles -- Top-Down Protein Sequencing (ESI and MALDI) -- Peptide mapping and screening • Full suite of ESI and MALDI data processing tools plus UV and FLD support; includes the complete ProteinScape functionality, e.g. -- Protein database searches -- Glycan database searches -- Glycopeptide analysis...