timsTOF , MALDI Guided SpatialOMx Innovation with Integrity

MALDI Guided SpatialOMx The tumor microenvironment is a highly variable ecosystem, giving it an intrinsic temporal character. De-coding the cellular communication within the tumor microenvironment via label-free MALDI Imaging and x-omics promises to improve the understanding of the mechanisms responsible for drug resistance and augment the precision of histological diagnoses. timsTOF fleX harnesses the power of SpatialOMx to deliver maximum intelligence per pixel. Use the timsTOF fleX to dive deeper into the tumor microenvironment and beyond. timsTOF fleX combines the best x-omics platform with...

Intelligent Pixels Maximize Intelligence per Pixel An average cell measures approximately 10 μm in diameter. Using MALDI guided laser microdissection (LCM) for example, a 50 μm LCM tissue section will contain roughly 25 cells; enough for bottom-up proteomics analysis on the timsTOF fleX. One instrument that gives you the capability to do both – high spatial resolution, high speed MALDI and high sensitivity ESI analysis. Ultimate flexibility and specificity at the click of a software button, comes standard on the timsTOF fleX. SpatialOMx Workflow SpatialOMx is the combination of using MALDI Imaging...

Why choose Bruker? timsTOF fleX – The best 4D x-omics and MALDI Imaging system Since introducing the refleX MALDI-TOF system in 1992, Bruker has continuously pushed technological boundaries, developed a wide variety of applications, and became the uncontested MALDI market leader. In MALDI Imaging, spectra are collected spatially, creating a mass spectrum at every location which can be projected as a 2D map. Single datasets may contain hundreds to thousands of unique, label-free ion images, which can be used for molecular marker discovery or investigating molecular content of specific regions....

Bridging the gap between 4D X-Omics and Pathology 1 Within Tissues Strict tissue-specific protein expression is uncommon, however, some types of proteins are predominant - higher molecular weight motor proteins in muscle, smaller neuropeptides within the brain, digestive enzymes within the gastrointestinal tract, transport proteins within the kidney and barrier function-related proteins within the skin. The proteome of each tissue or organ points to its primary function. For Regulation of Protein Expression and Celluar Processes Within any given cell, the processes that regulate life, growth,...

1 Brain A highly complex and energy-intensive organ, coordinated higher functions, e.g. motion, perception, and cognition, are received, processed and executed in the brain. Neural proteins show specific expression patterns among cells and structures, as well as in subcellular structures such as axons, dendrites, and synapses. 2 Lung The lungs are primarily responsible for respiration: the gaseous exchange of O2 and CO2 between air and blood occurs in ~300 million alveoli. Pneumocytes, bronchial epithelium, and the endothelial cells facilitate O2 /CO2 exchange, while alveolar macrophages protect...

Trust the Experts Years of defining the leading edge for technology in MALDI imaging gives Bruker the largest imaging customer base packed with reference leaders in a wide variety of research fields. Learn what some of them have to say about how the timsTOF fleX enables SpatialOMx as an essential innovation for molecular imaging. Prof. Richard R. Drake, Director, Proteomics Center, Medical University South Carolina, USA “he timsTOF fleX is an innovative instrument that synergizes multiple analytical T capabilities to allow development of novel omics workflows. For imaging MS, it may be potentially...

4D X-Omics Workflows − Just a Click Away Label-free quantitation on the timsTOF fleX with PASEF: investigation of proteomic changes in tissue samples of mouse gastric carcinoma. The timsTOF fleX offers a combination of two unique technologies, Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and Parallel Accumulation Serial Fragmentation (PASEF) to improve ion utilization efficiency and data acquisition speed. The performance of the timsTOF fleX mass spectrometer with PASEF for label-free quantitation of proteins can be demonstrated on proteins extracted from sectioned mouse...

SpatialOMx Automated Molecular Annotation Workflow SCiLS Lab - Industry Leading Imaging Software For SpatialOMx a new mass spectrometry imaging workflow with automatic metabolite annotation will be supported by SCiLS Lab and MetaboScape: regions of interest can be transferred from SCiLS Lab to MetaboScape and the annotated peak lists can be loaded into SCiLS Lab for visualization of spatial compound distribution. • Vendor-neutral analysis and visualization • Quantify target molecules directly from tissue • New SpatialOMx workflow for automatic metabolite annotation timsTOF fleX data Import spectral...

timsTOF  MALDI Guided SpatialOMx timsTOF fleX uniquely enables SpatialOMx PASEF powered LC-MS/MS identification matched with spatial localization identifies and locates multilevel genomic expression in tissue without labels timsTOF fleX provides results without compromise All the 4D OMICS power that you demand from proven PASEF workflows with fast, software-controlled changeover to MALDI for rapid molecular imaging For research use only. Not for use in clinical diagnostic procedures. Bruker Daltonik GmbH Bremen · Germany Phone +49 (0)421-2205-0 [email protected] - www.bruker.com Scan the...