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ETD II Unparalleled Versatility - Small Molecules to MegaDalton Complexes Innovation with Integrity

maXis II - Market Leading High-Resolution LC-QTOF This heralds a new era in QTOF technology with unprecedented performance across wide-ranging applications to resolve the most demanding analytical challenges. maXis II delivers a full range of market leading performance parameters simultaneously. Bruker’s Ultra-High Resolution QTOF technology has reached new heights in accurate-mass LC-MS/MS. In addition, the system offers Electron Transfer Dissociation (ETD) capabilities for sequence analysis of intact proteins including monoclonal antibody subunits. Furthermore, the High Mass Option...

Innovations Market leading innovations with the latest in Bruker‘s QTOF technology, already proven on impact series Ultra High Time-of-Flight resolution across wide m/z range acquired at LC time scales Enhanced Dynamic Range not limited by space charge effects Versatility Versatility from Small Molecules up to MegaDalton complexes Full scan MS, DIA - broad band CID, DIA- middle band CID, CID MS/MS, and LC-ETD MS/MS on a single instrument Productivity Fast time-to-results Dedicated biopharmaceutical and protein analytics software solutions for greater productivity and increased confidence...

maXis II -Technological Innovations Resolution that enables the separation of 34S isotope separated from 13C isotope at m/z 311 acquired at a scan speed of 12 Hz. Versatility and Research Performance Easily switch between the following workflows: - Small molecule - Electron Transfer Dissociation - Native MS without Hardware changes super molecular complexes Reagent anion source with

80,000 FSR (Full Sensitivity Resolution) achievable from 1- 50 Hz acquisition speed to separate near isobaric peaks Ultra-reliable mass stability from dalton to megadalton Native Mass Spectrometry enabled without sacrificing other performance vectors – for structural investigation of large proteins complexes sprayed in their native state Deconvoluted spectrum of Adalimumab Heavy Chain clearly enabling the reliable determination of the correct monoisotopic mass Electron Transfer Dissociation (ETD) for MS/MS of Intact Proteins and Identifications of Modifications High resolution reectron –...

Certainty in Small Molecule Analysis # possible formulae Fast, Accurate Identification of Unknowns Bruker’s unique and integrated hardware and software combine to rapidly and precisely identify molecular species. The superb high quality data from the maXis II, i.e. accurate mass and true isotopic patterns (TIP), in combination with Bruker’s unique ab initio formula discovery tools, SmartFormula™ and SmartFormula 3D™, provide accurate molecular formulae with a maximum level of certainty. Mass Accuracy low ppm accuracy plus Chemical Expertise additional confidence plus SmartFormula (Mass...

Achieve Knowledge Faster with Innovative Software Bruker’s application-specific software platforms convert the high quality data from the maXis II into knowledge. SmartFormula for Unambiguous Determination Compass Open Access - Simplicity with Certainty SmartFormula permits the unambiguous elemental composition determination by combining accurate mass and true isotopic pattern information. Deliver an expert analytical result first time for every walkup lab user, independent of their knowledge or experience. Compass OpenAccess puts the most sophisticated mass spectrometry tools at the...

A New Level of Confidence: The Benefits of Enhanced Resolution, TIP and Mass Precision middle-down and bottom-up ESI and MALDI mass spectrometry techniques“ Ayoub Daniel, Elsa Wagner-Rousset, Beck Alain, et al. mAbs 2013; 5:5, 699–710 Enhanced resolution and mass accuracy allows the complete characterization of closely related heterogeneities within the antibody subunit. Read more in: “Correct primary structure assessment and extensive glyco-profiling of cetuximab by a combination of intact, middle-up, Adalimumab Light Chain, 23 kDa measured in LC - Time-Scale Mr '23397.6208 variable region...

Fast and Confident Characterization Light Chain Heavy Chain LC-MS chomatogram of reduced antibody Adalimumab from 28 to 48 minutes Building on the powerful subunit workflows already accessible, the maXis II with its increased resolving power of 80,000 enables the measurement of the monoisotopic mass of antibody heavy chains in a simple reduction experiment, without time-consuming enzymatic digestion, minimizing the possibility of introducing artifacts. Mass Spectrum of Heavy Chain with resolved isotopes Class leading performance allows faster and more confident characterization of the heavy...

Sequence Verification by Top-Down Top-down molecular weight measurement plus LC-ETD-MS/MS sequence data helps to detect and localize modifications in monoclonal antibodies much faster. workflows including acquisition, data processing and reporting for sequence verification and ready detection of modifications can be achieved using Biopharma Compass software. This software fully automates LC-ETD-MS/MS workflows resulting in sequence verification and identification LC-ETD chromatogram of Carbonic Anhydrase LC-ETD spectrum of Carbonic Anhydrase (29 kDa) obtained using new ICC algorithm (ion...

Intact Protein Mixture Analysis 117 LC-MS run of serum sample Utilizing well-established protein and peptide analysis functionalities, Bruker‘s bioinformatic software provides a sophisticated tool for further data interrogation of intact protein profiles and top down protein sequencing studies. > 30 proteoforms in this peak from 7 to 46 kDa co-eluted in a 6 seconds window Mr '16580.0519 Sub-ppm mass accuracy & isotopic accuracy Isotopic resolution achievable for proteins > 40 kDa MS/MS available for the ID of the proteoform of interest

Dynamic Source Configuration In addition to the ESI sources, Bruker‘s life sciences MS systems support a wide range of source options from Bruker and from third-party vendors, all switchable within seconds. CaptiveSpray™ nanoBooster The CaptiveSpray nanoBooster is the ion source that brings your MS to the next performance level. The operation is just as easy as performing electrospray. The nanoBooster provides robust quantitation, enhanced glycoanalysis and supercharging. Atmospheric Pressure Photo Ionization is used for less polar or non-polar molecules that cannot be ionized in either ESI...