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Native MS Solutions scimaX MRMS: Perfectly designed for Native MS

Native MS Solutions scimaX MRMS: Perfectly designed for Native MS
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Native MS Solutions scimaX MRMS: Perfectly designed for Native MS - 1

Native MS Solutions • scimaX MRMS: Perfectly designed for Native MS Mass Spectrometry Innovation with Integrity

Native MS Solutions scimaX MRMS: Perfectly designed for Native MS - 2

scimaX Ion Path – Perfectly Balanced for Native MS The critical requirement for native MS is an optimal pressure gradient between the electrospray source and the mass analyzer. The ion path on the scimaX MRMS (magnetic resonance mass spectrometry) enables the transmission of intact biomolecular complexes into the ParaCell, allowing for extreme resolution analysis. Now fragile fragment-protein, protein-substrate, multi-protein biomolecular complexes, membrane proteins with nanodiscs, mAbs, and native protein top-down analysis can all be analyzed on a standard (unmodified) scimaX. Standard...

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Native MS Solutions scimaX MRMS: Perfectly designed for Native MS - 3

Native MS of Protein Complexes (15T MRMS) 20* (D) Successful analysis of large proteins and their complexes requires three key conditions to be met. First, the proteins must be transmitted and detected without breaking intramolecular bonds. Secondly, the protein must be sufficiently desolvated to allow observation of the free protein ions. Finally, the extraordinary amount of charge on the molecules must not overwhelm the detector leading to space charge or coalescence issues. The scimaX and ParaCell technologies were designed with these goals in mind and are proven in labs worldwide for...

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Native MS Solutions scimaX MRMS: Perfectly designed for Native MS - 4

Native MS Applications for Fragment Based Drug Discovery MRMS takes on the challenge of discovering new drugs for Malaria Malaria causing, crescent shaped Plasmodium falciparum gametocyte in blood smear Advancing the frontiers of Fragment-Based Drug Design (FBDD), Professor Ron Quinn and co-workers of the Griffith Institute for Drug Discovery, analyzed a specially curated natural products fragment library of over 600 unique compounds against 62 separate potential malaria drug protein targets.[1] Using MRMS, 79 fragments were identified interacting with 31 proteins and later shown to have in...

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Native MS Solutions scimaX MRMS: Perfectly designed for Native MS - 5

The scimaX MRMS is built on conduction cooling technology. It does not require liquid cryogens or a vent line for operation. Six Proven Reasons for Using the scimaX MRMS for Native MS 1 Ion path leaves fragile non-covalent interactions intact Bruker's perfectly balanced MRMS ion optics have always been the most efficient in the industry for native MS - no changes to vacuum or a separate, special instrument needed. Complexes are desolvated but not disrupted. 2 Only instrument to offer broadband extreme resolution Only Bruker MRMS offers the ability to detect molecules at 200 m/z at 20...

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Native MS Solutions scimaX MRMS: Perfectly designed for Native MS - 6

Proven MS technology for routine analysis of native protein complexes Wongkongkathep P Han JY, Choi TS, Yin S, Kim HI, Loo JA. (2018) Native Top-Down Mass Spectrometry and Ion , Mobility MS for Characterizing the Cobalt and Manganese Metal Binding of α-Synuclein Protein, Journal of the American Society for Mass Spectrometry, 10.1007/s13361-018-2002-2, 29 (9), 1870–1880 Vu H, Pedro L, Mak T, McCormick B, Rowley J, Liu M, Quinn RJ (2018) Fragment-Based Screening of a Natural Product Library against 62 Potential Malaria Drug Targets Employing Native Mass Spectrometry, ACS Infectious Diseases,...

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