Benchtop MALDI-TOF neofleX MALDI-TOF More insight per pixel Groundbreaking 116 protein panel at 30 µm imaging in 7 hours Innovation with Integrity

If you could simultaneously image >100 proteins on a tissue section, how would you ask questions differently? Methods that track targeted protein expression on tissue surfaces have significantly changed the understanding of biology and disease. The ability to connect data from single cell, or bulk experiments to specific locations within the tissue microarchitecture informs deep insight on how cellular neighborhoods interact to drive biological processes and disease states. Understanding complex cellular landscapes that exist for certain disease pathologies, such as the tumor microenvironment,...

The advanced technology built into neofleX™ is accessible to anyone utilizing pre-run automation, powered by SCiLS™ autopilot, which automatically optimizes the instrument prior to each acquisition to ensure peak performance for each tissue section or tissue microarray (TMA). neofleX leverages the advanced MALDI HiPLEX-IHC workflow for spatial biology that uses unique peptide reporters for each probe, meaning you retain the familiarity and ease of IHC with the flexibility of mixing and matching any validated probes and panels without modifications. Sample Integrity Tissue samples are not degraded...

Bring OMICS context to cancer research Integrating neofleX into your spatial biology workflow allows you to know both where your proteins are and what they are doing. Multiomics goes beyond RNA and protein expression to redefine important inputs by spatially co-localizing selected proteins with glycans, targeted metabolites and lipids to expression targets within the tissue or tumor microenvironments. Absolute intensity Mean spectrum of the entire N-glycan MALDI Imaging dataset from a colorectal cancer section and multichannel ion image showing the distribution of the indicated peaks (blue triangles...

Automated acquisition setup Automated SCiLS lab data import and pre-processing Automated image extraction and OME-TIFF export l^yK ATfttsi' SCiLS Scope visualization of MALDI HiPLEX-IHC data from a colorectal cancer sample. Various cancer-, immune-, and microenvironment-specific markers were activated, and the distribution of the proteins is shown (A . In the zoomed window(B) the distance measure was applied to highlight blood vessel diameter. Prof. Dr. Carsten Hopf Hochschule Mannheim - Center for Mass Spectrometry and Optical Spectroscopy neofleX presents an incredibly versatile opportunity...

World class MALDI lineup The leading expert in MALDI neofleX Multiomic translational ™ MALDI-TOF MS: For over 30 years, matrix assisted laser desorption ionization (MALDI) TOF MS has proven its analytical capability. Offering unparalleled speed (time to results) and flexibility, MALDI-TOF MS stands out for its low sample volume requirements and ability to handle a broad range of sample content, including salts and buffers. rapifleX® Targeted throughput COOH COOH Time of flight principle Kinetic energy of an ion acceleration by voltage U: Ekin = 1/2 mv2 = zeU Velocity of an ion in a field free...

Innovation with Integrity timsTOF fleX Flexible discovery Powerful analysis, fast results With continued innovation, intuitive software and seamless automation enhance the depth of MALDI analyses while ensuring high throughput solutions. scimaX® Toughest problems Conventional steel targ target r et Apply sample Localized, concentrated sample spots Smart consumables IntelliSlides® – Maximize information content per pixel with intelligence IntelliSlides are the perfect tool for SpatialOMx®. They simplify your MALDI Imaging workflows by enabling automated setup of each measurement and via barcoding...

A protein primary sequence is a critical attribute directly impacting its structure and function. As such it is an essential parameter to verify, whether working on affinity-based reagents for biological assays or a biological drug. The neofleX brings high performance MALDI top-down sequencing (MALDI-TDS) to benchtop instruments for the first time, enabling users with a powerful sequence validation tool for purified proteins that minimizes the time to results. MALDI-TDS measurements can be carried out in less than a second and produce singly charged spectra which can easily be analyzed with Bruker's...

Comprehensive toolbox for biologics characterization x10 5 Ubiquitin from 2.4 fmol to 2.4 pmol on spot NISTmAb heavy chain (2+) G1F High sensitivity and resolution across a wide m/z range provide essential data for biologics projects MALDI offers the unique capability of measuring biopolymers across a wide molecular weight range with high speed, sensitivity and non-overlapping charge states (1+ dominated spectra). This provides the foundation for a large range of workflows. The simple output and rapid analysis make MALDI attractive for applications such as clone screening or enzyme activity assays....

First Bruker commercial MALDI system Caprioli et al publishes first imaging paper - access - clean - operate 2015 solariX with dual source Bruker commits to development of MALDI Imaging on ultrafleX II scimaX launches rapifleX® launches

■ True pixel imaging: Patented smartbeam 3D technology delivers industry-standard 20 |jm spatial resolution for detailed analysis. ■ Enhanced imaging detectors: Capture high-quality data for accurate results. ■ Sweeping MS/MS capabilities (LID, details. Seamless top-down and bottom-up sequencing of proteins. Effective flight length Benchtop size (LxWxH) 124 cm linear mode 260 cm reflector mode Powerful tools, seamless integration ■ IntelliSlides®: Maximize insight per pixel with intelligent slide technology. ■ SCiLS autopilot: SCiLS™ Lab and open-source OME-TIFF files directly after measurement...