Designed for Accuracy High resolution quantitative proteomics Innovation with Integrity

Setting New Standards in Accuracy The development of mass spectrometry based proteomics approaches has dramatically improved researchers‘ capabilities of characterizing the protein content of biological systems, while simultaneously offering information on proteins identity, abundance and modifications. Accurate and reproducible protein quantitation is key for success and Bruker’s latest UHR-QTOF platforms are designed to provide the best performance in this area. The Instant Expertise™ acquisition methods provide easy and straightforward access to the underlying biology. Generic quantitation...

Accuracy = Resolution x Sensitivity Full Sensitivity Resolution (FSR) In traditional QTOF architecture, high resolution is obtained at the price of ion losses through slits or multiple reflections and the maximal sensitivity is therefore not accessible at full resoultion. The Bruker FSR architecture, in contrast, makes use of the maximum number of ions to obtain the highest resolution available without sensitivity loss. This ensures the best possible selectivity and sensitivity, at any speed. Whatever strategy is chosen for quantitative mass spectrometry based analysis of complex proteomics samples,...

Repeatable High Performance The impact ll's unique combination of sensitive, high resolution and high dynamic range spectra opens a new dimension in MS based quantitation. Both label-free quantitation workflows and quantitation based on chemical labels are very easy to implement with pre-set methods. Preservation of spectral quality in MS/MS mode guarantees optimal performance for these data dependant acquisition (DDA) approaches. Instant Expertise™ The impact ll's default ID/Quant acquisi- tion method ensures accurate MS based quantitation with regular spacing of MS The number of MS/MS events...

Across the Whole Range The impact II allows targeted proteomics experiments to be run in either an MS based format or at the MS/MS level based on data independent acquisition (DIA). Superior resolution and full sensitivity of MS and MS/MS spectra facilitate both approaches on Data aquisition in MS mode for targeted peptide and protein quantitation provides accurate results over a large dynamic range based on high resolution extracted This mode is applicable to both high and low complexity samples and both approa- ches benefit from the uncompromised resolution and sensitivity of the impact II....

Label-Free Intact Protein Analysis Peptide based quantitation is a very sensitive approach but it is blind to some essential biological events such as protein proteolysis. By combining Full Sensitivity Resolution with the True Isotopic Pattern (TIP™) capability of Bruker’s UHR-QTOF systems, accurate measurement of intact proteins is readily available, even in complex mixtures. The Dissect™ and SNAP™ algorithms extract the accurate mass and intensity information to perform a direct label free approach on intact proteins. Dissect™ Dissect™ processing of an E.Coli lysate separated with a 20 min...

Label-Free Shotgun Proteomics Accuracy of the label free quantitation (ID/Quant) workflow Thanks to the combination of very stable nano electrospray (CaptiveSpray™) and advanced QTOF technology, the impact II offers robust and accurate label- free quantitation. Since this strategy relies on every single peptide precursor ion that is detected at the MS level, the benefit of high resolution over the entire mass range directly translates into superior ratio Peak intensity Quant accuracy over a large dynamic range spread over L.FQ intensity sample 1 (toglDj 500 ng of yeast digest were spiked with...

Verified by Science The impact II provides latest Bruker UHR-QTOF technology The unique combination of quantitative accuracy, dynamic range and reproducibility make the system capable of delivering quantitative data with best accuracy. Full-Sensitivity-Resolution Technology • 50,000 resolving power at MS and MS/MS level without compromising sensitivity or acquisition speed • Highly accurate extracted ion chromatogram (1-2 mDa) CaptiveSpray nanoBooster Source • Zero adjustment for excellent reproducibility • Acetonitril dopant for improved sensitivity Collision Cell 50 Gbit/s Detection System...