spotOn™ • Accelerate Protein and Biopharmaceutical Characterization Innovation with Integrity^-

Simple, Reliable LC-MALDI Preparation Delivering samples ready for MS analysis Guided Control – from vial to target spot. The spotOn wizard leads you step-by-step through the workflow, from autosampler to the full LC-separation spotted to the MALDI target Operational ease – redefined workflows make the use of the spotOn easy and intuitive, saving time and improving reproducibility. Built in hardware features including double independent concentric needles (DICN™) and a PowerWash™ ensure safe sample handling, and the optional barcode reader easily transfers pre-existing sample information through...

Weeks of ESI data analysis can save you hours of MALDI work spotOn helps accelerate time consuming protein characterization workflows Extending the peak capacity of standalone MALDI methods by HPLC enhances the natural attributes of MALDI mass spectrometry. Improved spot purity enhances the leading top-down sequencing performance and further improves the detection of traditionally hard to detect ions such as crosslinked, glycosylated or hydrophobic peptides. • igh order structure analysis – LC-MALDI MS allows the use of unconventional proteolytic H enzymes (such as pepsin) and the automatic determination...

nanoElute LC and spotOn status are displayed in one software interface A truly hyphenated technology, the LC and spotOn provide a concise overview of the progress of separation and preparation. The workflow is completed with (optional) matrix and calibrant addition within the spotOn, providing a sample plate ready for MALDI-MS and MS / MS analysis on rapifleX, ultrafleXtreme and autoflex series instruments References [1] Wiesner J et al. Expert Rev Proteomics. 2015; 12(2):115-23 [2] Ayoub D et al. MAbs. 2013;5(5):699-710 [3] Resemann A et al. MAbs. 2016;8(2):318-30 [4] Kohlstaedt M et al. MBio....