• A Comprehensive Screening Solution for Forensic Toxicology Innovation with Integri

TargetScreener Get the Complete Picture Forensic laboratories are frequently required to perform comprehensive screening of complex samples to identify drugs and other toxicants. This is a very challenging task due to the enormous number of analytes covered, their wide range of concentration and the complexity of sample matrices tested. Within the field of forensic toxicology, there is high requirement for analytical certainty in the accuracy and completeness of the results, so as to ensure that they will stand up to scrutiny in a court of law. In recent years, owing to the high level of specificity...

TargetScreener Workflow Prior to analysis, depending on the analytical requirements, blood serum or urine samples are prepared using LLE, SPE or protein precipitation. These are injected onto a reverse phase binary gradient UHPLCQTOF system for fragmentation, detection and confirmation. The Bruker TargetScreener employs a powerful and specific form of data acquisition called broadband CID (bbCID). This mode of data acquisition when used with the TargetScreener accurate mass forensic database and associated data mining software brings the full power of exact mass screening to the forensic laboratory....

The Benefits of Broadband CID (bbCID) Data Acquisition This method of data acquisition ensures all compounds eluting have molecular ion data and fragmentation spectra acquired all of the time – hence no compounds are missed! This is achieved by continuously cycling between TOF-MS and bbCID MS/MS resulting in the seamless collection of a comprehensive catalogue of precursor and fragment ion data in a single analysis. Collision cell rapidly alternates between low and elevated collision energies Q1 allows all ions to pass Collision cell TOF-MS Detection bbCID MS/MS Confirmation Fig.2: Schematic...

b) bbCID spectra, Qualifier Ions circled in green Fig.3: a) Chromatographic overlay of bbCID qualifier (QI) ion traces Fig.4: Broadband CID (bbCID) data acquisition differentiating two isomeric synthetic cannabinoids, JWH-122 and JWH-019. Their unique bbCID qualifier ions (highlighted in the green boxes) are used for unambiguous identification.

TargetScreener Applications Kit The TargetScreener applications kit incorporates a forensic toxicology accurate mass database detailing the exact mass of molecular ions, adducts, fragment ions as well as retention times that have been collected using an optimized UHPLC gradient to maximize confidence in identification. This highly curated database is key to enabling accurate and sensitive detection and identification of relevant compounds. Also included within the applications kit are the analytical and guard columns, a test mix and all the necessary chromatographic and mass spectrometric parameters...