timsOmniTM An eXtreme leap in deep proteoform sequencing and advanced structural elucidation Innovation with Integrity

An eXtreme leap for electron-based fragmentation techniques Welcome to the timsOmniTM , a transformative instrument poised to revolutionize mass spectrometry in systems biology. Designed to push the boundaries of scientific discovery, the timsOmni achieves extraordinary sensitivity, advances deep proteoform sequencing and delivers unprecedented precision in structural elucidation. Discovering the intricacies of proteoform diversity and gaining new insights into the functional plasticity of proteins is now within reach. The timsOmni further enables detailed structural elucidation of potential...

Experience unmatched MS and MSn sensitivity Powered by proprietary omni-directional lossless transfer and ion enrichment, signals for even the lowest-abundance ions are dramatically amplified. Through precise modulation of selected ion packets, any ion, regardless of its intensity, can be selectively targeted for electron-based fragmentation. Electron source Q8 omni-directional MSn ion enrichment and optical access Q5 omni-directional accumulation with trapped eXd Q2 omni-directional accumulation with resonance excitation CID and MSn isolation R Omni-directional MSn eXd with ion enrichment 1...

Advanced structural elucidation and deep proteoform sequencing by MSneXd coupled to the power of TIMS. • TIMS separation to determine conformational heterogeneity and CCS to reveal structure • Trapped eXd for optimal precursor utilization boosting fragment ion yield • Precise control of electron-based fragmentation for detailed molecular profiling • Omni-directional MSnand trapped eXd with ion enrichment for unmatched sensitivity Transfer multipole •— • Utilization of all PASEF modes for bottom-up proteomics and multiomics Explore theconformational landscape Collision induced unfolding (CIU)...

Nomenclature for the primary fragment ions types observed for peptides and proteins in tandem mass spectrometry. In ECD, when a protein or peptide ion captures a free electron, bond cleavage occurs rapidly and locally at the N–Cα bond, producing primarily c/z fragments. This non-ergodic process preserves labile post translational modifications like phosphorylation, glycosylation, sulfonation, etc. Combinations of ECD and CID using MSn eXd can be used to further enhance protein sequence coverage through formation of a/x, b/y and c/z ions. In CID, ions are gradually heated through multiple collisions,...

Precise modulation of electron kinetic energy and reaction time Precisely tune electron energy to access diverse fragmentation regimes. Adjust eXd d reaction times and explore uncharted electron energy levels for different classes of analytes to achieve optimal product ion coverage. eXd Landscape electron ionization Access to different electron-based fragmentation regimes becomes available by fine tuning electron energy from 0.1eV to >100 eV niECD electron capture Electron energy optimization – Electron Capture Dissociation – hot Electron Capture Dissociation – Electron Excitation Dissociation...

Powerful multimodal and multi-stage MSn Online multimodal fragmentation Multimodal fragmentation relies on combining information from first-generation fragment ion types, produced by complementary ion dissociation methods, to drastically increase sequence coverage. MULTI-STAGE MSn ION ACTIVATION NETWORK MS2 CID – Collision-cell Collision Induced C Dissociation CID – Resonant-excitation Collision Induced R Dissociation ECD – Electron Capture Dissociation EID – Electron Induced Dissociation PD – Photo-dissociation Successive stages of ion activation enable first- and second-generation fragments...

Charge DDA (cDDA) enables on-the-fly charge-state deconvolution during LC-MS analysis, allowing precise targeting of coeluting proteoforms across a wide dynamic range and eliminates redundant fragmentation of highly abundant species. By dynamically shifting the isolation window to non-overlapping regions of the m/z spectrum, cDDA significantly reduces chimeric spectra. • Ultra-fast on-the-fly charge-state deconvolution with intelligent m/z selection criteria. • Precursor selection targets nonoverlapping charge states producing high fidelity MS2 data. • Dynamic precursor accumulation optimizes...

Dynamic precursor accumulation for superior LC-MS/MS sensitivity Top-down and middle-down LC-MS approaches enable rapid analysis of recombinantly expressed biologics. Targeted methods are particularly advantageous when high specificity and maximum sensitivity are required for protein sequencing, whether in complex matrices or purified samples typical of biopharmaceutical workflows. To further advance such analytical strategies, dynamic precursor accumulation actively regulates ion flow in MS/MS scans to optimally fill and compress the ion cloud within the trapping sections of the Omnitrap platform....

Sequencing Antibody Complementarity-Determining Regions Analyzing and monitoring circulating antibody levels is critical for characterizing the progression of a disease, identifying patients with delayed symptom onset and predicting potential longterm immunity. • Complementarity-Determining Regions (CDRs) are the hypervariable loops within the variable domains of the light and heavy chains primarily responsible for selectivity and affinity towards a specific antigen. Comprehensive ion sequence ladders are essential to characterize the unique CDRs. Protein centric ECD analysis of a therapeutic...

Monitoring in vivo biotransformation improving the efficacy of biotherapeutics products for Multispecific antibodies are rapidly emerging as a leading class of biotherapeutics, capable of simultaneously binding to multiple target antigens. These innovative modalities offer novel mechanisms of action, with enhanced efficacy, reduced risk of resistance, and fewer side effects compared to traditional therapies. However, unlike monoclonal antibodies, multispecifics are more prone to degradation in vivo, requiring thorough metabolite characterization. Top-down and middle-down MS approaches are particularly...